Sequences from confirmation amplicon


Confirmation sequences for line 156F09 and hit At1g06450 are shown.
Line ID 156F09
Locus At1g06450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:1965655
Primers used for PCR NR32 - 8474, show primer details
   
Sequence Name 18-K114404-0022-156-F09C-oriL
GenBank Accession LN494406 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K114404-0022-156-F09C-oriL
AATATATCCTGAAAACCGTCATGATCAACACCGCGTTAAAATTAATTGGAGAGCAAGCTA
TTAGCTTAAGCTATGTATCTTTAATTAATTAGAAAAAATCCGAGAAAAAAGGAAACTCAG
GTACACGAATTTATTTAAGAAAAGGATAAATACTCTCTTTTTTTTTATAAAGAACCCTAA
TGCTCTCACACAATCAATCAAACCAAAAAGACTTCTCGTATTCATGCCTCTCCCTCCTAA
GAAAAGAACCCTAATTTTTATTATCTGCAGAAATCATCATCATGAACGAGATTCATCAAC
CGCTGGCGAGGAGAGTATGGAGAAGCAACGTCGATGAAGAGATGGCTCGTATGGCCGAAT
GTCTCAAGCGATTCCCACTCATCGCTTTCGATACAGAGTACCCTGGCATTATTTTCCGCA
CTTATTTCGATTCCTCTAGCGACGAAGGTTACAGAGCCATGAAAGGTAATGTCGAGAACA
CCAAGCTGATCCNGTGTGGTTTCATCTTATTCA
   
Sequence Name 40-K114405-0022-156-F09C-NR32
GenBank Accession LN503792 [GenBank]
Sequencing primer gene specific primer, NR32: 5'-GTTGAAAAGAGTGAAACCACACTG-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K114405-0022-156-F09C-NR32
CTTTCAGGCTCTGTACATTCGTCGCTAGAGGAATCGAATAAGTGCGGAAAATAATGCCAG
GGTACTCTGTATCGAAAGCGATGAGTGGGAATCGCTTGAGACATTCGGCCATACGAGCCA
TCTCTTCATCGACGTTGCTTCTCCATACTCTCCTCGCCAGCGGTTGATGAATCTCGTTCA
TGATGATGATTTCTGCAGATAATAAAAATTAGGGTTCTTTTCTTAGGAGGGAGAGGCATG
AATACGAGAAGTCTTTTTGGTTTGATTGATTGTGTGAGAGCATTAGGGTTCTTTATAAAA
AAAAAGAGAGTATTTATCCTTTTCTTAAATAAATTCGTGTACCTGAGTTTCCTTTTTTCT
CGGATTTTTTCTAATTAATTAAAGATACATAGCTTAAGCTAATAGCTTGCTCTCCAATTA
ATTTTAACGCGGTGTTGATCATGACGGTTTTCAGGATATATTCAATTGTAAATGGCTTCA
TGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCCACAAAATTTT

Last Updated on 06.12.2018 16:11