Sequences from confirmation amplicon


Confirmation sequences for line 316F11 and hit At1g07160 are shown.
Line ID 316F11
Locus At1g07160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:2199141
Primers used for PCR DL65 - 8474, show primer details
   
Sequence Name 32-K018512-022-316-F11C-DL65
GenBank Accession LN500384 [GenBank]
Sequencing primer gene specific primer, DL65: 5'-GCCACAGAGAAACAGAGAAAGACT-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K018512-022-316-F11C-DL65
ATATTCAAGCCTCTTTCTTCTCTCTCGTTGACACTAAACAGAGAAACACAAACGTCTAGA
AGACTAACAATCAAATCAATCATCAGAAAAAGGAAACTTTTAAGTTTTCTACGAAACGAT
GTCGTCTTCAGTTGCCGTTTGCAACTCACCGGTGTTCTCTCCGTCTTCGTCTCTTTTCTG
CAACAAACCGTTGAACACTTCTCCGGCACATGAAACCCTAACCCTGTCTCTTTCCCATCT
CAATCCTCCCGTTTCTTCTACTTCTCCTTCCGCCGCGTCTCCCACATCTCCGTTCTGCCT
CCGTCTCCTGAAACCGCCGGCTAAACTAGGGTTTGGATCGGACTCTGGTCCGGGAAGCAT
TCTGAAGAGGAAACGACCGACGACGCTTGATATACCGGTGGCTCCGGTTGGTATTGCAGC
TCCTATATCGAATGCAGATACGCCGAGGGAGGAGAGTAGAGCAGTGGAGAGGGAAGGTGA
TGGTTACTCTGTTTATTGCAAGAGAGGTAAAAGAGAAGCTATGGAGGATCGTTTCTCTGC
CATCACTAATCTTCAAGGAGATCCCAAACAGGTAATTTCAATTTTCTTCCGTAATTTCGA
GTTTATTTACTGATTATTGCTTAATTAGTATGAATTGCGATTTGAAGATGGGTAATCAAT
TGATATTAAACATGTAAATGAGTATATGAGTAAATGGCTTCATGTCCGGGAAATCTACAT
GGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTT
TTTCAATTTCAAAAA
   
Sequence Name 33-K018512-022-316-F11C-8409
GenBank Accession LN500802 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K018512-022-316-F11C-8409
AAGCCATTTACTCATATACTCATTTACATGTTTAATATCAATTGATTACCCATCTTCAAA
TCGCAATTCATACTAATTAAGCAATAATCAGTAAATAAACTCGAAATTACGGAAGAAAAT
TGAAATTACCTGTTTGGGATCTCCTTGAAGATTAGTGATGGCAGAGAAACGATCCTCCAT
AGCTTCTCTTTTACCTCTCTTGCAATAAACAGAGTAACCATCACCTTCCCTCTCCACTGC
TCTACTCTCCTCCCTCGGCGTATCTGCATTCGATATAGGAGCTGCAATACCAACCGGAGC
CACCGGTATATCAAGCGTCGTCGGTCGTTTCCTCTTCAGAATGCTTCCCGGACCAGAGTC
CGATCCAAACCCTAGTTTAGCCGGCGGTTTCAGGAGACGGAGGCAGAACGGAGATGTGGG
AGACGCGGCGGAAGGAGAAGTAGAAGAAACGGGAGGATTGAGATGGGAAAGAGACAGGGT
TAGGGTTTCATGTGCCGGAGAAGTGTTCAACGGTTTGTTGCAGAAAAGAGACGAAGACGG
AGAGAACACCGGTGAGTTGCAAACGGCAACTGAAGACGACATCGTTTCGTAGAAAACTTA
AAAGTTTCCCTTTTTCTGATGATTGATTTGATTGTTAGTCTTCTAGACGTTTGTGTTTCT
CTGTTTAGTGTCAACGAGAGAGAAGAAAGAGGCTTGAATAT

Last Updated on 06.12.2018 16:11