Sequences from confirmation amplicon


Confirmation sequences for line 869G12 and hit At1g07270 are shown.
Line ID 869G12
Locus At1g07270 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:2231713
Primers used for PCR HV34 - 8474, show primer details
   
Sequence Name 95-K033100-022-869-G12C-8409
GenBank Accession LN515326 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>95-K033100-022-869-G12C-8409
ATAAGGACTCCTCGGTCTTTTGTGATTAAGTAATCCATCTCATCTGCAATTATTAACCTG
GAAAAGAGTTTGAATTAAGATAACACTGCCTTGTTTCAGAAATATTGTTATGTAATCAAA
GCACAACAACCGTACATCATTCTTGAGCTGGAAGATTCTTGTTTTTGAGAAAACAAATTC
TGAAGATGTTGTAGAGGTGAAGAGTTGGTGTTAGCATTCTTGCCAGGCTTGATTTCACCA
AGAATCTAACAACAAAATAAGCCTATAAGCATCAATATTAAACTGAAATCAAGAACTCTA
AGGTCTATAGTTATACCTTGCTGAAAATATCTGTTGTTTTACTCAGCGACGTGCAATTCA
CAGACAATGTATCCACTGGTGGCAAACCTGCCTGATAAAATGCACATNGGTTCAA
   
Sequence Name 47-K033100-022-869-G12C-HV34
GenBank Accession LN506112 [GenBank]
Sequencing primer gene specific primer, HV34: 5'-ATTGAACATTGGTGCATTTTATCA-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K033100-022-869-G12C-HV34
TGTCTGTGATTGCACGTCGCTGAGTAAACAACAGATATTTTCAGCAAGGTATAACTATAG
ACCTTAGAGTTCTTGATTTCAGTTTAATATTGATGCTTATAGGCTTATTTTGTTGTTAGA
TTCTTGGTGAAATCAAGCCTGGCAAGAATGCTAACACCAACTCTTCACCTCTACAACATC
TTCAGAATTTGTTTTCTCAAAAACAAGAATCTTCCAGCTCAAGAATGATGTACGGTTGTT
GTGCTTTGATTACATAACAATATTTCTGAAACAAGGCAGTGTTATCTTAATTCAAACTCT
TTTCCAGGTTAATAATTGCAGATGAGATGGATTACTTAATCACAAAAGACCGAGGAGTCC
TTTATGATCTTTTCATGCTGCCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAG
AAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTT
ATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGG
CGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACA

Last Updated on 06.12.2018 16:11