Sequences from confirmation amplicon


Confirmation sequences for line 669B05 and hit At1g08480 are shown.
Line ID 669B05
Locus At1g08480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:2684841
Primers used for PCR MJ30 - 8474, show primer details
   
Sequence Name 44-K029916-022-669-B05C-8409
GenBank Accession LN505068 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K029916-022-669-B05C-8409
AGAAAAACCAAAATAACCAAAACCAAACAGTTCAGTTTTTGCTAGAGTTTTTTAAAGCCA
TCCGACGACGAAGCAGTGGGCTTAGTCCTCTAGTATCTGTGCTTCCAAGGACTAAAGCCA
GAAAAACCTAGCCTAGTGGAAACTTTATTATATCAAAAAGTAACAGAAACTTTCCAATGG
AAAATCAAATGAAGAAGAAAAAAATAAACCGATGACAGCAGAGTAAAATCGAGTACCAGC
CGAGTCATAGATAATGGACCAACAATATCTATAGATTCCAAAACACAGAGCGAGAGAAAG
ATAGAAG
   
Sequence Name 44-K029916-022-669-B05C-MJ30
GenBank Accession LN505069 [GenBank]
Sequencing primer gene specific primer, MJ30: 5'-ACCCACCGTAATTTCCTTCTATCT-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K029916-022-669-B05C-MJ30
TAGATATTGTTGGTCCATTATCTATGACTCGGCTGGTACTCGATTTTACTCTGCTGTCAT
CGGTTTATTTTTTTCTTCTTCATTTGATTTTCCATTGGAAAGTTTCTGTTACTTTTTGAT
ATAATAAAGTTCCACTAGGCTAGGTTTTTCTGGCTTTAGTCCTTGGAAGCACAGATACAA
GAGGACTAAGCCCACTGCATCGTCGTCGGATGGCATAAAAAACCTAGCAAAAACTGAACT
GTTGGTTTGGTATTTGGTATTCTTACTGAAGATCTTACCAAACGTAAGATCAATACAAAT
GGAAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAA
GTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAAC
AAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTC

Last Updated on 06.12.2018 16:11