SimpleSearch - Line and FST details
Line specific information
| Line ID | 669B05 |
| Vector Used | pAC161 |
| Line Availability | available as T3 set from NASC (N464145) |
| Segregation Analysis | 50:50:49 |
| Confirmed for Hit | At5g08650 At1g08480 |
| Parent of DUPLO pair | 2011 |
| Parent of pair(s) | none |
Gene hit At4g24710
| Sequence (A. th genome BLAST matches underlined) | >34-K024027-022-669-B05-8409 CGTCGCTAAACCCAATCTTCCTCGTTGCCTCCACGAAAACAAATTCCTCGTATCTGGTAT GGGATCCTCCCTATAGTGAGCCGGCATGGGATCCTCCCTATAGGGA |
| GenBank Accession | BX894556 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr4:12749160 - go to primer design |
| BLAST e Value | 4e-15 |
| Hit Clone Code (BAC ID) | F22K18 |
| Hit Gene Code | At4g24710 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | pachytene checkpoint-like protein |
| Insertion Classification | 5' region |
| Confirmation Status | unknown |
Gene hit At5g08650
| Sequence (A. th genome BLAST matches underlined) | >34-K023058-022-669-B05-8409 TTGCAGATGCCAGGGTCAGGAGAGACAATAACACATTATAGCAGACAGGCAGAAAGCTCT TCACCTGGTTACGAGGAAGCTACCCCTATGGCGCTCCCTGTCCTATATCCCCCCCACCCG GCCCTCCTCCCGTGCTCCTTCCTCCGAACCCCCCCCCTTTCACTGCCTCCCTGATGCGCA TATCCCCCCCCTGCCCCCCCCCCCCCGGCTACTCATTCCCTCCCTCCCTCCACCCTCCCC CCACTCTCTCCTCCCCCCTCCCCCCCGCCTTCCCCCCCTCCTTCTCTCCCTTTTCTTCGC CATCTCCCCTTCCCTTCCTCGCCCCCCCCCCCCCCCCCGCGCTACGTGTGAGCGGCCGTG ACCCGCGCGTCACCACCCTCCCCTCCTGCCCCTCGTCCTTCCCCCCCCCCCCCCCCCTCC TCCTTCTTCCTCTTATG |
| GenBank Accession | BX660943 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr5:2809806 - go to primer design |
| BLAST e Value | 2e-32 |
| Hit Clone Code (BAC ID) | MAH20 |
| Hit Gene Code | At5g08650 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | Small GTP-binding protein |
| Insertion Classification | CDSi |
| Confirmation Status | confirmed, show confirmation sequences |
| Primer and wt-amplicons | show primer details |
Gene hit MUL3
| Sequence (A. th genome BLAST matches underlined) | >34-K022930-022-669-B05-8409 ATTGAATATATACTAACATTGAATAAAAGTTTGGCGTTAAGTGTATGGGGATTACTCCCT ATAGGGGAGGAGAAGTGGCGCCCCGTCACCCAAAGGATGCGCCCGATCTCTATGCTTCTC TGTACGTCGGCAGCTGACCCCTCCACTCACAGCTGAGATGTGCACTACAGTGATCGGCGG AGCCGGCGACGGAGTTGTGCAAAAGAGCAGAATATCTATCGTGAAGGGAGCCGTTACTTC ATTCCCCTTATTATACTAAAATCCCCTCACTTTGAGACATTGCCTCCTGTGGACCTGTTC CCCGATCCGCTACGCATTAGTTGAATCCGCGCGTCCTTAAATGCCTAGTTCCATCTCACT GCCGACCCTTCCATATATACACTTATAACCTCACAATGTCTCCCCCGTGAGCCATAGGTC AGTCAGTCCAAGATGAAAAGGCTCCAACACAGCAATCATTCTCTGAGACCCTTTCTCCGC AATCTTCCC |
| GenBank Accession | FR814315 [GenBank] |
| Graphic View |
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| Predicted Position of Insertion | Chr5:23117993 - go to primer design |
| BLAST e Value | 0.001 |
| Hit Clone Code (BAC ID) | MUL3 |
| Insertion Classification | Genome Hit |
| Confirmation Status | unknown |
Gene hit At1g08480
| Sequence (A. th genome BLAST matches underlined) | >34-K022987-022-669-B05-8409 ATTGATCTTACGTTTGGAAAGATCTTCAGTAAGAATACCAAATACCAAACCGCCAGTTCA GTTTTTGCTAGGTTTTTTATGCCATCCGACGACGATGCAGTGGGCTTAGTCCTCTTGTAT CTGTGCTTCCAAGGACTAAAGCCAAAAAAACCTATACTAGGGGAACTTTATTATATCAAA AAGTAACAGAAACTTTCCAATGGAAAATCTAATGAAGAAGAAAAAAATAAACCGATGACA GCAGAGTAAAATCGAGTATGGGATCCTCCCTATAGTGAGANCNNNCNNTCCGNGGANTCT CTCCCAGATGAAAAAATTTACGATAATTTGAAATACCTTTTTTA |
| GenBank Accession | BX660942 [GenBank] |
| Graphic View |
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| Predicted Position of Insertion | Chr1:2684839 - go to primer design |
| BLAST e Value | 7e-94 |
| Hit Clone Code (BAC ID) | T27G7 |
| Hit Gene Code | At1g08480 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | succinate dehydrogenase subunit |
| Insertion Classification | CDSi |
| Confirmation Status | confirmed, show confirmation sequences |
| Primer and wt-amplicons | show primer details |
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Last Updated on 10.06.2021 13:37 |
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