Sequences from confirmation amplicon


Confirmation sequences for line 054C05 and hit At1g08820 are shown.
Line ID 054C05
Locus At1g08820 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:2822457
Primers used for PCR BE32 - 3144, show primer details
   
Sequence Name 45-K028748-022-054-C05C-3144
GenBank Accession LN505412 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K028748-022-054-C05C-3144
GAAATCCCAGTTTTCTTTTGCCATCCTTCGAACGAAATTCGTAGCTATCTTTAGTTCGCC
TTTCAAGTTCATTAGCAGTATAATCGATCTTCCACAAAATCCACCGCGGCCATTTTTGGT
GTCTTTAACTTCCTTTGCATTCGTCGGCTGCCTTTAACTCATCATGTCCAACCATCCTAG
GCTCTTTTACAATCTCACCCTCCTAGAAAAATAAATTTTGAGAAATAAACAAATAACTAC
AAGAAAAATCCCTATTGCACCCAATAACTGTGATTATAAGCTAAGCTAAAATGAACTTGC
TGGAACATAACTACACTCACTGCTTGGCAAATTACTCTTCATCTTACTAACAAATCATAA
AAGTTGTTGCGAAAAGGGGTAAATACTCTTCTTACATATTGTGGCGGAGCTAGGGTCTCT
GACTGGCTATATAAACGATCCTTGAGGATGGAATCTTCAAATACTGCTCCCTGCTTCGGT
GTATTGATTGGAGATAGTTCAGGTGAGTCAGAAGGCGGTACGAGCGTGACCCTGAGTTTG
TTCTCCTC
   
Sequence Name 45-K028748-022-054-C05C-BE32
GenBank Accession LN505413 [GenBank]
Sequencing primer gene specific primer, BE32: 5'-GAAGGCAAACACATAGAGGAGAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K028748-022-054-C05C-BE32
ATCAATACACCGAAAGCAGGGAGCAGTATTTGAAGAATTCCATCCTCAAGGATCGTTTAT
ATAGCCAGTCAGAGACCCTAGCTCCGCCACAATATGTAAGAAGAGTATTTACCCTCTTTT
CGCAACAACTTTTATGATTTGTTAGTAAGATGAAGAGTAATTTGCCAAGCAGTGAGTGTA
GTTATGTTCCAGCAAGTTCATTTTAGCTTAGCTTATAATCACAGTTATTGGGTGCAATAG
GGATTTTCTTGTAGTTATTTGTTTATTCTCAAAATTTATTTTTCTAGGAGGGTGAGATTG
TAAAAGAGCCTAGGATGGTTGGACATGATGAGTTAAAGGCAGCCGACAATGCAAAGGAGT
TAAAGACACCAAAAATGGCCACAGTGGATTTTGTGGAAGATCGATATACTGCTAATGACT
TGAAGGCAACTAAAGATAGCTACGATTCATCGAGGATGGCAAAAGAAACTGGATTCAAAC
ACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCT
CCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTG
CGTCATCCCTTACGTCAGTGGAGATATCACATCAATCCACTTGCTTTGAAGACGTGGTTG
GAACGTCTTCTTTTTCCACGATGCTCCTCG
Primers used for PCR BE31 - 8474, show primer details
   
Sequence Name 84-K028753-022-054-C05C-8409
GenBank Accession LN514369 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>84-K028753-022-054-C05C-8409
TCAAAATTTTAAAAATCCATAACTTTCCGTGCTATATGTAACTGTGCTAAAGAGTTTAAA
TCTGGTGCAGGCTGATTTCAACCATTTTCGAAGCTTAATGGAGGAGCGATCT
   
Sequence Name 84-K028753-022-054-C05C-BE31
GenBank Accession LN514370 [GenBank]
Sequencing primer gene specific primer, BE31: 5'-TATGTTGGGAGCTTATAGATCGCT-3'
Sequence (A. th genome BLAST matches underlined) 
>84-K028753-022-054-C05C-BE31
TAGCACGGAAGTTAAGGAATTTAAAATTTGATAAGCAGAACCCGTTTTAACTTGGATTCA
AGAGCATCCACTTTCAGCTTCATCTCCTCAATGTCTTTAACCAACTTAAGTTCATCCAAT
CCTCTGACATTCTGGCCATCTGTTTTCTGCAGCTGGACAACATCTCTCTCCTTAGATATC
TTTGGTTCACTGTCAACAGCTATTCCATTAGTAAACCCTTGATCCCTAGGTAGATCCATG
GCCTTCTTCATGGGAGCGTCCAAATTAGTTGTTGCCTTAATCGCCCTTCCATCATCCGCA
TCCTTAACCGTATTTCCGGACATGAAGCCATTTAAACTATCAGGATATATTCAATTGTAA
ATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAG
AAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTT
ATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGG
CGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTC

Last Updated on 06.12.2018 16:11