Sequences from confirmation amplicon


Confirmation sequences for line 259H04 and hit At1g09490 are shown.
Line ID 259H04
Locus At1g09490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:3065467
Primers used for PCR 35ys - 8474, show primer details
   
Sequence Name 32-K122558-0022-259-H04C-35ys
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 35ys: 5'-CTACTGGTTAAGTGTTGCCTGTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K122558-0022-259-H04C-35ys
GTGGATTCGTGGATACAGTCTTGATCATTGAATCCAATTACCATTGGATTACTGGAGTTT
GAATTCATATGGTCATAATGTCATATGACCACTCGTTTATTCCTTGTACCAATTGACTTG
ACGACATCATCACCTAGCGTTACAAGTAAACGTTAAGTGATATTATTAGCTTAGTTTAGA
CACTATGTATCCAAACCTTAATTAGTGACAAATTTAGCCTAAACAATAATTATATATTAT
TGTTTATAAAATGCTTAGATTTTAAAGAGTCTCAACAACTTAAACCACTTGAATTAGTAA
TACTAACAAAATAAAAATACACATAATAGTTACAAATATACGAACAGAATCATAAAAGAC
ATTTCTCCTTGAGGCTAACAATAGTGTCTCTAAGGCTTGATTTCATAGGAGTGAACTCTA
CTCCCAAATTTTTCACTTTCTCCACACACACTTGGCATATCATCTCATTCATCTCACTTT
CTTCATTCCTAACAAAATCATCAACAAGTAAGTTTTTCAAAATCTTTCATTAAATTTATA
AACTTCATAAGATGATAAGCAAAGGTAGAGCTAGGGAAAGAGATCTCACGTATCAGCAAT
AGACAAATCTGGGAACAGCTTCCGAAGAATGTCTATAATGTCGTTTACACTCATATTTGG
ACCATCGATGATGTATCTGCCATTGGCTGAAGGAGTCTACAGGATATATTCAATTGTAAA
TGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGA
AAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAA
   
Sequence Name 16-K122557-0022-259-H04C-oriL
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K122557-0022-259-H04C-oriL
TCATTGAATATATCCTGTAGACTCCTTCAGCCAATGGCAGATACATCATCGATGGTCCAA
ATATGAGTGTAAACGACATTATAGACATTCTTCGGAAGCTGTTCCCAGATTTGTCTATTG
CTGATACGTGAGATCTCTTTCCCTAGCTCTACCTTTGCTTATCATCTTATGAAGTTTATA
AATTTAATGAAAGATTTTGAAAAACTTACTTGTTGATGATTTTGTTAGGAATGAAGAAAG
TGAGATGAATGAGATGATATGCCAAGTGTGTGTGGAGAAAGTGAAAAATTTGGGAGTAGA
GTTCACTCCTATGAAATCAAGCCTTAGAGACACTATTGTTAGCCTCAAGGAGAAATGTCT
TTTATGATTCTGTTCGTATATTTGTAACTATTATGTGTATTTTTATTTTGTTAGTATTAC
TAATTCAAGTGGTTTAAGTTGTTGAGACTCTTTAAAATCTAAGCATTTTATAAACAATAA
TATATAATTATTGTTTAGGCTAAATTTGTCACTAATTAAGGTTTGGATACATAGTGTCTA
AACTAAGCTAATAATATCACTTAACGTTTACTTGTAACGCTAGGTGATGATGTCGTCAAG
TCAATTGGTACAAGGAATAAACGAGTGGTCATATGACATTATGACCATATGAATTCAAAC
TCCAGTAATCCAATGGTAATTGGATTCAATGATCAAGACTTGAACCACGTAATCCACCCT
TATCCTTAGAAGCTCATAAATATCACTAAAGGGACAGGCAACACTTAAACCAGTA

Last Updated on 06.12.2018 16:11