Sequences from confirmation amplicon


Confirmation sequences for line 144G09 and hit At1g09920 are shown.
Line ID 144G09
Locus At1g09920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:3226535
Primers used for PCR KB54 - 3144, show primer details
   
Sequence Name 26-K100851-0022-144-G09C-3144
GenBank Accession LN497839 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K100851-0022-144-G09C-3144
TCCACTATCCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGAC
AGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTCTCGATGGATGGATTAGT
GATATACAATCTTTTTTTCTAAAAGAGTTCATTTTTATTTGGTCACCTACTAAAAGGTTG
ATTCTTTTTTGTTACTTTGCTTAAAGTCCTTCATGATAAATCTATTGATTATGTATCAGC
TTCCTGGTGTCTGTACAAGAGTTTTATTGTCTTCTGTTTATCGTCATGCTTTCTACGATT
TGTCTGATGGTTTGCTTAGTTTCTAAACTTTCTAAAGATATATGTGTATGCTTTGTCTAG
TTATTGTTGTGTTGAGTCTGCTGAGGCTGGAAATTTTTGAATGCCCTAATTCTTATTTGG
GATCAGTAAATCTAATCCCTTGTGTATGTTCAGTGGTAGCATTTACCTGGATGGTTGATC
CCATGAGACTGATTATCTTAATGTTTTCAGTGACAGAGACATTCCATCACTGAATATCGA
TCTGCATCGTGTACATTGTGCTCGGAATTTAGAAAAATGTAAGATTTGTGGTGATATGGT
ACCCAAAAAGCATGCTGAGGAACACTACTTGA
   
Sequence Name 26-K100857-0022-144-G09C-KB54
GenBank Accession LN497840 [GenBank]
Sequencing primer gene specific primer, KB54: 5'-GGAGCATGTGTGTTCAAGTAGTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K100857-0022-144-G09C-KB54
TATCACCACAATCTTACATTTTTCTAATTCCGAGCACAATGTACACGATGCAGATCGATA
TTCAGTGATGGAATGTCTCTGTCACTGAAAACATTAAGATAATCAGTCTCATGGGATCAA
CCATCCAGGTAAATGCTACCACTGAACATACACAAGGGATTAGATTTACTGATCCCAAAT
AAGAATTAGGGCATTCAAAAATTTCCAGCCTCAGCAGACTCAACACAACAATAACTAGAC
AAAGCATACACATATATCTTTAGAAAGTTTAGAAACTAAGCAAACCATCAGACAAATCGT
AGAAAGCATGACGATNAACAGAAGACAATAAAACTCTTGTACAGACACCAGGAAGCTGAT
ACATAATCAATAGATTTATCATGAAGGACTTTAAGCAAAGTAACAAAAAAGAATCAACCT
TTTAGTAGGTGACCAAATAAAAATGAACTCTTTTAGAAAAAAAGATTGTATATCACTAAT
CCATCCATCGAGAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTC
TCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGT
GCGTCATCCCTTACGTCAGTGGAGATT
Primers used for PCR FK20 - 8474, show primer details
   
Sequence Name 47-K101269-0022-144-G09C-8409
GenBank Accession LN506145 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K101269-0022-144-G09C-8409
GCCATTTACATTGAATCCCTAGTTATACTTGTAATCACCGACACAAACCGTGGAACCAAA
CTGAATCATACAGCTCACGAGTATCTTTAATGTGACACATATTCTCAATTGCAATCACCG
ATCAATTCCAAAATTGTTATAACCTCGAGTCCAGAATATCGAATTACACAAGTCATCATC
GAACGATTACGCAACAAAGAGAAAGCAACCAATCGCCGAAAAAAACTATGATCAAATAGA
AACAAGTCAACAACCACAGACAGAGAAATCAATGGTTTTAGGGGGGAAACAAACCAGTGG
TTGCAGACGATGGTGATTTCTCCAGTCGCAGTCTCCATCGTCAAACGAGAAACAAACTTC
TATTTCGAAGCAAATAACAGATCAGAGGAGAACCAGGACGATCAGAGACCTGAAGAAACG
AAATTTCAGA
   
Sequence Name 47-K101275-0022-144-G09C-FK20
GenBank Accession LN506146 [GenBank]
Sequencing primer gene specific primer, FK20: 5'-TTCTGAAATTTCGTTTCTTCAGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K101275-0022-144-G09C-FK20
TTGCTTCGAATAGAAGTTTGTTTCTCGTTTGACGATGGAGACTGCGACTGGAGAAATCAC
CATCGTCTGCAACCACTGGTTTGTTTCCCCCCTAAAACCATTGATTTCTCTGTCTGTGGT
TGTTGACTTGTTTCTATTTGATCATAGTTTTTTTCGGCGATTGGTTGCTTTCTCTTTGTT
GCGTAATCGTTCGATGATGACTTGTGTAATTCGATATTCTGGACTCGAGGTTATAACAAT
TTTGGAATTGATCGGTGATTGCAATTGAGAATATGTGTCACATTAAAGATACTCGTGAGC
TGTATGATTCAGTTTGGTTCCACGGTTTGTGTCGGTGATTACAAGTATAACTAGGGATTC
AATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCA
ATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAAATG

Last Updated on 06.12.2018 16:11