Sequences from confirmation amplicon


Confirmation sequences for line 169C08 and hit At1g11360 are shown.
Line ID 169C08
Locus At1g11360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:3822634
Primers used for PCR 53ze - 3144, show primer details
   
Sequence Name 66-K124919-0022-169-C08C-53ze
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 53ze: 5'-CATCATCTGCAAGATTTAGTACGG-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K124919-0022-169-C08C-53ze
TATCTGCCACATACGAAGCCAATACCATGCTTCATTGTGCTTTGAGTTCCTGAACTCATC
CCCTGAAATTGCATCACTGGATTTCTATGGCCTTACCAAATATATACTTACTTATAATTC
TCGAAAAACTTCAGATATGCTGAAACAATTCCAGGCACTCGTAAGAAGCACAACGGATCC
CAACGCTAAAGGTTCCTACCATGTATGTGCAGAAATGTTCGACAAAGCTACTGATTGTTT
TGATGACGCCTTCACAACTTTGGCATCCAAGGACTATATCACTTTAGAACAAAGGGTTGT
TTGTACGTTTGACATGGGGGATGGGTGTAAAGAAGAGCTGTTAACTTTCACTCCTAGCCC
ACAACTCTTCAAAGATGTTTCTATTGTCAAAAATCTTTCTTCTATGGTTTTGGTGATTCT
AGATTATTATCTGAAAAAATAAATAAAAGTTATTTTAGTTTTGGTATAGTGTGTTTTAAT
TATATAATTAGAATGACTATATATTACCATTGGAGAATATCTATGGTTTTGGTTATATAT
TTTTACAGCCGTTAGATCTTCAAACCGATCAATCTAACGGTTCTTAACAATATCCAATTC
TCATAAGAAAAGAGTACAAAAATAAAAAGAGTCGCTGTCACTATACCGTACCGTACAGAG
TCAATTGAAGAAACCGAGAGAGAGAGTGAGCGGAATCATGACTTCTCCGGGAAAATCTCC
GAGATCCGACCGGAAATCACCGACGGTTGTCACCGTCCAGCCGTCTTCTCCCAGATTCCC
GATCAGCACACCTACTGCTGGAGCTCAACGTAAGATCGGGATCGCTGTCGATCTAAGCGA
CGAGAGTGCGTACGCAGTTCAATGGGCTGTTCAGAACTATCTCCGATCAGGTGACGCCGT
TGTTCTCCTTCATGTTCAACCGACGAGTGTACTTTACGGCGCCGATGGGGGCGCTATCGG
TT
   
Sequence Name 50-K124918-0022-169-C08C-3144
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K124918-0022-169-C08C-3144
CCTTCGCAGACCCTTCCTCTATATAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCC
GGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGAAAACCGATAG
CGCCCCAATCGGCGCCGTAAAGTACACTCGTCGGTTGAACATGAAGGAGAACAACGGCGT
CACCTGATCGGAGATAGTTCTGAACAGCCCATTGAACTGCGTACGCACTCTCGTCGCTTA
GATCGACAGCGATCCCGATCTTACGTTGAGCTCCAGCAGTAGGTGTGCTGATCGGGAATC
TGGGAGAAGACGGCTGGACGGTGACAACCGTCGGTGATTTCCGGTCGGATCTCGGAGATT
TTCCCGGAGAAGTCATGATTCCGCTCACTCTCTCTCTCGGTTTCTTCAATTGACTCTGTA
CGGTACGGTATAGTGACAGCGACTCTTTTTATTTTTGTACTCTTTTCTTATGAGAATTGG
ATATTGTTAAGAACCGTTAGATTGATCGGTTTGAAGATCTAACGGCTGTAAAAATATATA
ACCAAAACCATAGATATTCTCCAATGGTAATATATAGTCATTCTAATTATATAATTAAAA
CACACTATACCAAAACTAAAATAACTTTTATTTATTTTTTCAGATAATAATCTAGAATCA
CCAAAACCATAGAAGAAAGATTTTTGACAATAGAAACATCTTTGAAGAGTTGTGGGCTAG
GAGTGAAAGTTAACAGCTCTTCTTTACACCCATCCCCCATGTCAAACGTACAAACAACCC
TTTGTTCTAAAGTGATATAGTCCTTGGATGCCAAAGTTGTGAAGGCGTCATCAAAACAAT
CAGTAGCTTTGTCGAACATTTCTGCACATACATGGTAGGAACCTTTAGCGTTGGGATCCG
TTGTGCTTCTTACGAGTGCCTGGAATTGTTTCAGCATATCTGAAGTTTT

Last Updated on 06.12.2018 16:11