Sequences from confirmation amplicon


Confirmation sequences for line 699E04 and hit At1g12080 are shown.
Line ID 699E04
Locus At1g12080 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4084886
Primers used for PCR 06tj - 8474, show primer details
   
Sequence Name 36-K116785-0022-699-E04C-06tj
GenBank Accession LN502372 [GenBank]
Sequencing primer gene specific primer, 06tj: 5'-ACGCTCTAACCCGAGTAGATTGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K116785-0022-699-E04C-06tj
ACTTCGTTTTGATTCAAATTTTCAAAACCAAGAATATTAGGTTTTTTTGCACTCAAGATT
GCATCATTGACGGATTGACCTCTTTAAATTTCTCTTAATTACTCCTCTTTGATGAAATGA
TCAAAATATTTATATTCATTGATTAGATTCTCTCGTCTTATTGTTAACGTTTACAGTTAA
TATGAAAAGTTCCAAATAAATGTACGATCTTATAGTATCTCTTAAGAAAACTTTCATCTG
TACTTATTTTTTTGAAAAACTGAATAATGTTATGTTGTATATTTAGAAAAAGGGATGTAA
TTTTTTATTTTTTTGCATGTAGACATAAACGGTTTTAATCTTCCATGTTCTCGGTTTAAG
TTTGTACTTTGTTGCTACGACCAAGCATGTAAACAATGTAGCAACCATTAAAAGATCCAA
ACATCTGATTTCATCATAACAAACCCAACAAACATAGAAAATACTTATGAAATGTTGATA
AAATAAAATAAACAGAAAACGATATGAGACCAAAAAAGGTCATATATAGCTTCTTCCTTC
CTCTTTAAAAGTGATCATTGGACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGA
AATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTA
CCAATTTTTTTTCAATTCACAAATGTAGATT
   
Sequence Name 17-K116784-0022-699-E04C-8409
GenBank Accession LN494023 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K116784-0022-699-E04C-8409
ATTTACATTGAATATATCCTGTCCAATGATCACTTTTAAAGAGGAAGGACGAAGCTATAT
ATGACCTTTTTTGGTCTCATATCGTTTTCTGTTTATTTTATTTTATCAACATTTCATAAG
TATTTTCTATGTTTGTTGGGTTTGTTATGATGAAATCAGATGTTTGGATCTTTTAATGGT
TGCTACATTGTTTACATGCTTGGTCGTAGCAACAAAGTACAAACTTAAACCGAGAACATG
GAAGATTAAAACCGTTTATGTCTACATGCAAAAAAATAAAAAATTACATCCCTTTTTCTA
AATATACAACATAACATTATTCAGTTTTTCAAAAAAATAAGTACAGATGAAAGTTTTCTT
AAGAGATACTATAAGATCGTACATTTATTTGGAACTTTTCATATTAACTGTAAACGTTAA
CAATAAGACGAGAGAATCTAATCAATGAATATAAATATTTTGATCATTTCATCAAAGAGG
AGTAATTAAGAGAAATTTAAAGAGGTCAATCCGTCAATGATGCAATCTTGAGTGCAAAAA
ACCTAATATTCTTGGTTTTGAAATTTGAATCAAACAAAGTTACTTACCACAGTTACCTTT
TTTTGTTGATTGACCAAGATTGTGGGCTTTTAATCTACAATCTACTC

Last Updated on 06.12.2018 16:11