Sequences from confirmation amplicon


Confirmation sequences for line 054A01 and hit At1g12680 are shown.
Line ID 054A01
Locus At1g12680 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4321667
Primers used for PCR PC29 - 8474, show primer details
   
Sequence Name 35-K103737-0022-054-A01C-PC29
GenBank Accession LN501804 [GenBank]
Sequencing primer gene specific primer, PC29: 5'-TCGCACTTTTCACTAGAGGGTTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K103737-0022-054-A01C-PC29
TGTCGAGTCTGTTGGTTCCTTTAGAGACGAATTGCTGGTGTTGCTACTGCACCTCCTTGT
GGTGCATCATCTTTGGTTTCGTCAGGAAGAGGTTTGAAGAGGAAGATAGGGTGCATAGAT
GTTTCCACACAGACTGGTAGGAAGAATAAAATAGACGATGACTATGTTTTTGGTCGAAAT
ATTGGGAAAGGGAAATTTGGGTCAGTCAGGATCTGTAAGTCTAGGAAGAATGGGACTGAG
TTTGCTTGTAAAACTCTGAAGAAGGGGGAGGAGACTGTTCACCGGGAAGTTGAGATAATG
CAACATTTGTCTGGTCATCCTCGGGTTGTCACATTGCATGCTGTGTATGAAGAGTCAGAC
TGTTTCCATCTTGTGATGGAGCTGTGTTCAGGGGGACGTTTGATTGATCAGATGGTAAAG
GTGGGCAGGTATTCTGAGCAACGAGCAAATGAAACCAATTATGATGTCCAACCACGTCAC
AAACACTGATAGTTTAAACCGTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGA
AATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTA
CCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 51-K103738-0022-054-A01C-8409
GenBank Accession LN507480 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K103738-0022-054-A01C-8409
TTACAATTGAATATATCCTGACGGTTTAAACTATCAGTGTTTGTGACGTGGTTGGACATC
ATAATTGGTTTCATTTGCTCGTTGCTCAGAATACCTGCCCACCTTTACCATCTGATCAAT
CAAACGTCCCCCTGAACACAGCTCCATCACAAGATGGAAACAGTCTGACTCTTCATACAC
AGCATGCAATGTGACAACCCGAGGATGACCAGACAAATGTTGCATTATCTCAACTTCCCG
GTGAACAGTCTCCTCCCCCTTCTTCAGAGTTTTACAAGCAAACTCAGTCCCATTCTTCCT
AGACTTACAGATCCTGACTGACCCAAATTTCCCTTTCCCAATATTTCGACCAAAAACATA
GTCATCGTCTATTTTATTCTTCCTACCAGTCTGTGTGGAAACATCTATGCACCCTATCTT
CCTCTTCAAACCTCTTCCTGACGAAACCAAAGATGATGCACCACAAGGAGGTGCAGTAGC
AACACCAGCAATTCGTCTCTTAAAGGAACCAACAGACTCGACAGCTTCAGTCTCCTTACA
CCGTTTCTTAAGTCTAGCATAACCCTCTAGTGAAAAAGTGCGA

Last Updated on 06.12.2018 16:11