SimpleSearch - Line and FST details
Line specific information
| Line ID | 054A01 |
| Vector Used | pAC161 |
| Line Availability | available as T3 set from NASC (N405089) |
| Segregation Analysis | 50:46:44 |
| Confirmed for Hit | At1g06260 At1g12680 |
| Parent of DUPLO pair | none |
| Parent of pair(s) | 90263, 90291, 90314, 90356, 90361 |
Gene hit F12K21
| Sequence (A. th genome BLAST matches underlined) | >01-K012480-022-054-A01-8409 TTTGATCCATGTAGAATTTCCCGGACATGAANGCCTTTACNCCATATAACAAGCTTCTTC ATGTTGACTAGTTACTAATCTTGTTCAATTTCATTTAAAAAATGCAATGCACCCGTTGTA TAAACGATTTTTTTTTGGTTGAATATAATTTTACATTATTTCAAAAAAAAATTTTTATCG AGTATAAATATGTCAAAAACATACATTTGTTAATCTCTTAAAATGTCAATATACATATTA TAAAAAGATTTTTTTTCAAACACTTGCACATTGAAAATAAAAACAATATTTTTTAAAAAC ACATTTTTTTCATACAAAAATTATTCACCCTTTGTAATAAATTTATTTTATAGATAAAAA AACTGCCATAGCTTTTTTAGTTTTGTATTTTTATTAAAGCTTTTGACCTAAGGGGATTTT AATTTGGTTTTTTTGTTTTTTCAAATATTTTTCTTTTAAATTTACATTTTTCTTGTTTAA ATATATATATCGCTTTTTTTTTTTTTTTTAAGGTTTTTAAAAAAAAAATTTTTTTTGATT TGAAAAACTTTTTTTACAAAGGGGTTTTTGGGGGGTTTTTTTTTTTTTATAATGGGGCTT AAAAAAAATTTAAAATTAAAAAA |
| GenBank Accession | AL754293 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr1:12625399 - go to primer design |
| BLAST e Value | 9e-79 |
| Hit Clone Code (BAC ID) | F12K21 |
| Insertion Classification | Genome Hit |
| Confirmation Status | unknown |
| Other FSTs Supporting this Hit | AL754290 [GenBank] AL754293 [GenBank] |
Gene hit At1g06260
| Sequence (A. th genome BLAST matches underlined) | >01-K012454-022-054-A01-35St TGACGTAAGGGATGACGCACAATCCCCTATCCTTCGCAGACCCTTCCTCTATATAAGGAA GTTCATTTCATTTGGAGAGGACAGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCG GTTTAAACTATCTGTGTTTGACAGGATATATTGGCGGTTAAACCTACGAGAAAAGAGCGT TCATTATAATAATCGGATATTTAAAAGGGCGTGAACTGGTTTACTCCGTTCGTCCATTTG TATGTGCATGCCAACCACAGGGTTCCCCTCGCGAGTGCTTGGCATTCCGTGCGATAATGA CTTCTGTTCATCCACCCTAACGTCCGATAGCCTGACGACGGAGCAGCATTCCAAAGAGAT CCCTTGGCTCGGCTGGGTCGGGTATGGGATCCTCCCTATAGTGAGTCGTTTAACGTAGGC ATCAACAAGATTAAAACAGGGAATTTAGTGTCTCTCTCAGAACAACAACTCATAGATTGT GATGTCGGGACTTACTACANAGGCTGTAGTGGCGGATTAATGGAAACAGCATTTGAATTC ATCAAAACCAAGGGGGGACTCGGCACGGGGAACCGACTACCCATACACAGGCATAGAGGG GGTCCTGCGACCAAGAAAAATCCAAAAATAAGG |
| GenBank Accession | FR801733 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr1:1917036 - go to primer design |
| BLAST e Value | 7e-89 |
| Hit Clone Code (BAC ID) | F9P14 |
| Hit Gene Code | At1g06260 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | Cysteine proteinases superfamily protein |
| Insertion Classification | CDSi |
| Confirmation Status | confirmed, show confirmation sequences |
| Primer and wt-amplicons | show primer details |
Gene hit At1g12680
| Sequence (A. th genome BLAST matches underlined) | >01-K012471-022-054-A01-8409 NTGATCCATGTAGAATTTCCCGGACATGAANGCCTTTACAATTGAATATATCCTGACGGT TTAAACTATCAGTGTTTGTGACGTGGTTGGACATCATAATTGGTTTCATTTGCTCGTTGC TCAGAATACCTGCCCACCTTTACCATCTGATCAATCAAACGTCCCCCTGAACACAGCTCC ATCACAAGATGGAAACAGTCTGACTCTTCATACACAGCATGCAATGTGACAACCCGAGGA TGACCAGACAAATGTTGCATTATCTCAACTTCCCGGTGAACAGTCTCCTCCCCCTTCTTC AGAGTTTTACAAGCAAACTCAGTCCCATTCTTCCTAGACTTACAGATCCTGACTGACCCA AATTTCCCTTTCCCAATATTTCGACCAAAAACATAGTCATCGTCTATTTTATTCTTCCTA CCAGTCTGTGTGGAAACATCTATGCACCCTATCTTCCTCTTCAAACCTCTTCCTGACGAA ACCAAAGATGATGCACCACAAGGAGGTGCAGTAGCAACACCAGCAATTCGTCTCTTAAAG GAACCAACAGACTCGACAGCTTCAGTCTCCTTACACCGTTTCTTAAGTCTAGCATAACCC TCTAGTGAAAAGTGCGACCTGAAGTTGGATGATCGTGATGCAGTTGAACATGATAAATCA TCTGGTAAATTCTCCCAACCCTCAGTTTCACTACCTTTCCGCTTCTTCCTCATCAAACCA GCCAACAGATAACGAATATCAGAATCACTTACATAAGAAAACAACAAGTCTTCTCCCTGC AAATCAGGACAAGCGGAATCATCATCAAAACCAACTGCAGAAACAAAATGCTATAGACCA ATCAAAAGTTTAGTACATAAAATGTAGCCCAAAAAGCCATTTTCCA |
| GenBank Accession | AL754292 [GenBank] |
| Graphic View |
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| Predicted Position of Insertion | Chr1:4321667 - go to primer design |
| BLAST e Value | 0.0 |
| Hit Clone Code (BAC ID) | T12C24 |
| Hit Gene Code | At1g12680 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | phosphoenolpyruvate carboxylase-related kinase 2 |
| Insertion Classification | CDSi |
| Confirmation Status | confirmed, show confirmation sequences |
| Primer and wt-amplicons | show primer details |
| Other FSTs Supporting this Hit | FR801732 [GenBank] |
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Last Updated on 10.06.2021 13:37 |
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