Sequences from confirmation amplicon


Confirmation sequences for line 395G09 and hit At1g12950 are shown.
Line ID 395G09
Locus At1g12950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4421044
Primers used for PCR JY34 - 8474, show primer details
   
Sequence Name 06-K037813-022-395-G09C-JY34
GenBank Accession LN488719 [GenBank]
Sequencing primer gene specific primer, JY34: 5'-TGTATGTATGGTGCCTTTAGCTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K037813-022-395-G09C-JY34
CGGACAGCGTTTGGAGCAGGGAAAGTATCAATGCTTGGCGTTTACTTACAACGCTCGTGG
GTTATTCTAAGCGTAACCGCTCTCTTCCTTTCTCTAATCTATATCTTCGCGGCTCCAATC
CTGACCTTTATAGGCCAAACCGCTGCAATCTCGGCCATGGCAGGGATTTTCTCCATTTAC
ATGATCCCACAAATCTTTGCCTACGCTATCAACTTCCCAACGGCGAAGTTTTTGCAATCA
CAAAGCAAGATCATGGTCATGGCTGGAATATCCGGTGTGGTTCTTGTGATCCATAGTTTC
TTCACGTGGCTAGTCATGTCTAGGTTACACTGGGGACTTCCTGGTTTAGCTTTAGTGCTT
AACACGTCGTGGTGGGTCATCGTTGTTGCACAACTTGTGTATATCTTTAATTGTACGTGT
GGAGAGGCTTGGTCTGGATTTACGTGGGAGGCTTTTCACAATTTGTGGGGGTTTGTTAAA
TTGTCTTCAAACCGAAGATCTGATCATATCCACCATATTGGTTATGGGCGTCAGCACATC
CCCCTTTCGTTTTAAAAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCA
ATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTC
AAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAA
ATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATC
TTTATTTCGACGTGTCTACAT
   
Sequence Name 14-K037813-022-395-G09C-8409
GenBank Accession LN492420 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K037813-022-395-G09C-8409
GCCATTTCAATTTTTAAACGAAAGGGGGATGTGCTGACGCCCATAACCAATATGGTGGAT
ATGATCAGATCTTCGGTTTGAAGACAATTTAACAAACCCCCACAAATTGTGAAAAGCCTC
CCACGTAAATCCAGACCAAGCCTCTCCACACGTACAATTAAAGATATACACAAGTTGTGC
AACAACGATGACCCACCACGACGTGTTAAGCACTAAAGCTAAACCAGGAAGTCCCCAGTG
TAACCTAGACATGACTAGCCACGTGAAGAAACTATGGATCACAAGAACCACACCGGATAT
TCCAGCCATGACCATGATCTTGCTTTGTGATTGCAAAAACTTCGCCGTTGGGAAGTTGAT
AGCGTAGGCAAAGATTTGTGGGATCATGTAAATGGAGAAAATCCCTGCCATGGCCGAGAT
TGCAGCGGTTTGGCCTATAAAGGTCAGGATTGGAGCCGCGAAGATATAGATTAGAGAAAG
GAAGAGAGCGGTTACGCTTAGAATAACCCACGAGCGTTGTAAGTAAACGCCAAGCATTGA
TACTTTCCCTGCTCCAAACGCTTGTCCGCATAGCGTTTCCAACGCACTTCCCATTCCAAG
CTAAA

Last Updated on 06.12.2018 16:11