Sequences from confirmation amplicon


Confirmation sequences for line 453H07 and hit At1g13820 are shown.
Line ID 453H07
Locus At1g13820 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4736528
Primers used for PCR JH15 - 8474, show primer details
   
Sequence Name 64-K101270-0022-453-H07C-8409
GenBank Accession LN511159 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K101270-0022-453-H07C-8409
TCGGACTGAGCCTTTACATTGTATGATCCATAGTTTTGGAAGTCTCATATTAAAAGGCCT
GTGGTTTTGGTTGGTCCGAGCCTCGGTGCTGCTGTCGCAATTGACATTGCGGTCAACCAT
CCAGAAGCGGTACTTATAGAATTCTTGAGGAGCTTTCATTATTTCTCATAAAGAAGTTTT
TAGTCAACATGTCAGAAGATATTGACTAGGAGAAGAAACTCATGAATATTCAGGTTGAGT
CCTTGGTTTTAATGGACGCAAGCGTTTACGCAGAAGGTACTGGAAACTTGGCAACGTTAC
CTAAAGCAGCAGCTTATGCTGGGGTAAGTTCTCATCAAGTTCTATACATAAAAGTCCCTT
TATTGTATTGGAGGATGATCCTCTTGAGTAAAACTCTTCACAATTCTGTTAACACTATGG
TTTTTTGGGTATATGAATTTGGGATTTTAGGTGTATCTTCTAAAGAGTATTCCATTACGG
TTATACGTTAACTTCATATGTTTCAATGGTATCTCATTGGAAACTAGTTGGGACTGGACA
AAGGTAAACAAGCCTTACTAAGTAATTCTTATTGGTAAACAAGAAACTAGCTCTCATCAG
TCTGATTTTTTTCGTCAATCTAGATGGGTCGCTTGCATTGTCTATATCCTTGGTGGGAAG
ATGCAACTGTTAGTTTTATGACTAGCGG
   
Sequence Name 64-K101276-0022-453-H07C-JH15
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, JH15: 5'-GTTACGTTGTATCCTCCGCTAGTC-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K101276-0022-453-H07C-JH15
AGGATATAGAAATGCAAGCGAGGAATCTAGATTG

Last Updated on 06.12.2018 16:11