Sequences from confirmation amplicon


Confirmation sequences for line 629A01 and hit At1g14240 are shown.
Line ID 629A01
Locus At1g14240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4866503
Primers used for PCR 32yq - 8474, show primer details
   
Sequence Name 41-K122488-0022-629-A01C-32yq
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 32yq: 5'-TGACTTTAGATGTACGTCCGGATT-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K122488-0022-629-A01C-32yq
GATCTGAATCTAGATGTTGACTTTGTAACATATTTTTAGACTTCAACTGTGGTATATGGT
CGAATTGTTAGTGATGACTATTTGTGCATATGTTTAGGTGACATTTGTGTCAAGTGAGCA
TGTGCCTCCTGAGTACTCGCGTACGATTGCCTACGGAAATATTTCATACACAATCTATAT
TCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
CCGCAACAATTATTAT
   
Sequence Name 10-K122487-0022-629-A01C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K122487-0022-629-A01C-8409
ACATTGAATATAGATTGTGTATGAAATATTTCCGTAGGCAATCGTACGCGAGTACTCAGG
AGGCACATGCTCACTTGACACAAATGTCACCTAAACATATGCACAAATAGTCATCACTAA
CAATTCGACCATATACCACAGTTGAAGTCTAAAAATATGTTACAAAGTCAACATCTAGAT
TCAGATCCAAATGTAGTATAGTTCTGTTGTTGTTAGTTTCTTAATCCGGACGTACATCTA
AAGTC

Last Updated on 06.12.2018 16:11