Sequences from confirmation amplicon


Confirmation sequences for line SALK_113003 and hit At1g15720 are shown.
Line ID SALK_113003
Locus At1g15720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5406128
Primers used for PCR 53ew - LBb3, show primer details
   
Sequence Name 12-K114334-SALK_113003C-53ew
GenBank Accession LN484618 [GenBank]
Sequencing primer gene specific primer, 53ew: 5'-TAAGAAACCTCTTTCGAGCTTCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K114334-SALK_113003C-53ew
TCTTTGAGAGCTGATTAAGATGAGAGATGGCATTGTACCTGATGTTAGTCTCACGAATCT
TCTGGTAAATCTCAGGTTCCTCAAACGCCTTGTTTAGATCACTCTCCCATTTCAACAACT
CACGAGTCACCAAGTCGCTCTCTTTCTCCTTCAATGTCCCAATCCGATTCCTCCAAATCC
TCTCAAGGGCATCACCGAAGAAGCCATCCGAAGCATTCACTGGCCACATAAACCTAAGCG
TGCATTCCACAGCAGTCCAGCAATACGCAGATTTGAGAGATCCCATGATAACCGAATGTT
CCTGTAACAGAAGCTTCTCAAGAATTTCGAGGAGATCAAGCATTCCTTCGTCTACGGTAC
CTTGAATCATGGAATTGGAGATATCCCGCANAACAGCAGTTAGCTTGAGTTTTACACAGT
CGTCGGAATCTACTGGTTTCAGAGCCGATNACAGTGGAAAAGCGTAAACCCTAGGATTTA
GTTGGCGTCGGAGGAAAAATTCAGCTACCCATTTGTCCATCACTTCCATTTCCAAGACTT
TGCAAGTAAAACTCATTGACGCTNAGACAACTTAATAACACATTGCGGACGTTTTTAATG
TACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGC
CNGGCCCTGAGAGAGTTGCAGCANGCGGTCCACGCTGGTNTGCCCCAGCAGGCGAAAATC
CTGTTTGAT
   
Sequence Name 02-K114333-SALK_113003C-R210
GenBank Accession LN484314 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K114333-SALK_113003C-R210
AGTCTTGGAATGGAAGTGATGGACAAATGGGTAGCTGAATTTTTCCTCCGACGCCAACTA
AATCCTAGGGTTTACGCTTTTCCACTGTTATCGGCTCTGAAACCAGTAGATTCCGACGAC
TGTGTAAAACTCAAGCTAACTGCTGTTTTGCGGGATATCTCCAATTCCATGATTCAAGGT
ACCGTAGACGAAGGAATGCTTGATCTCCTCGAAATTCTTGAGAAGCTTCTGTTACAGGAA
CATTCGGTTATCATGGGATCTCTCAAATCTGCGTATTGCTGGACTGCTGTGGAATGCACG
CTTAGGTTTATGTGGCCAGTGAATGCTTCGGATGGCTTCTTCGGTGATGCCCTTGAGAGG
ATTTGGAGGAATCGGATTGGGACATTGAAGGAGAAAGAGAGCGACTTGGTGACTCGTGAG
TTGTTGAAATGGGAGAGTGATCTAAACAAGGCGTTTGAGGAACCTGAGATTTACCAGAAG
ATTCGTGAGACTAACATCAGGTACAATGCCATCTCTCATCTNAATCAGCTTCTCANAGAG
CAGTGGGCACTACTTGGATGTTCTTCTCTTGAATCAGAAGCTCGAAAGAGGTTTCTT

Last Updated on 06.12.2018 16:11