Sequences from confirmation amplicon


Confirmation sequences for line 552C05 and hit At1g15950 are shown.
Line ID 552C05
Locus At1g15950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5479641
Primers used for PCR AC13 - 8474, show primer details
   
Sequence Name 30-K117337-0022-552-C05C-AC13
GenBank Accession LN499890 [GenBank]
Sequencing primer gene specific primer, AC13: 5'-TCTCGAGAGAGGTTACACAGTCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K117337-0022-552-C05C-AC13
GTCATTTTTATATTACTTTTTTGGTCACTTGTGATAATTATCTGAAGTTGTTGAGTGTTA
TTTTTATTGTTTTGATTGACAATTTGGATTTCTTTATAATTATTTTTCTTGCAGATGATC
CGAAGAACACACATTTGAGAGAACTAGAAGGAGGAAAGGAGAGACTGATTCTGTGCAAAG
CAGATCTTCAGGACTACGAGGCTCTTAAGGCGGCGATTGATGGTTGCGACGGCGTCTTTC
ACACGGCTTCTCCTGTCACCGACGATCCGGTGAGTCTTTTTCTTGGGTTTTCTATTTGTT
TTATCGCATTTTTAGTGGCTTACAGAGAGAATCGAATAAAGTTAAGGACCTACATGAAAT
AAAATAATACTTATATAGTAGTGGAGCTGATGATTTCATTTAGATTTTTCTGTCGTTTGT
TAATTAGACCATGTCGTGACGTTATCTAATCCCTTTTTTTAATCATGAACATAATATTTT
TTTTATCTATTTTTAAAATATCGCCTCATTAAAAGGGTGAAATCTCTAGCAATATTATTC
TTCATTGAATATTTGGTTTTTGCCAGTTTCGTCAAGTATTTTAATTTTAGGACGTTGAAA
GTTCCATCTTGTTGTGATGTTTTTATTACATTTGACTTTAAAATGAATATATTCAATTGG
TTATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
AGATGTCCG
   
Sequence Name 25-K117336-0022-552-C05C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K117336-0022-552-C05C-8409
TGACTGGAGGAGTATGACAAGTCTTGTTCAATTTTCAAACATATGTCTCGCAGTGATAAT
GGAACTACATTTGGTTATGCTCCAATTAGCGGTCTTGCTACCGTCAATCGAGAGATCCTT
ACCTGATGGTTGAATTTGATCTATCTTCTTGATGTTGTAAGACATATATATCAGCACACG
CTAATGGATCTTTCCTA

Last Updated on 06.12.2018 16:11