Sequences from confirmation amplicon


Confirmation sequences for line 155B11 and hit At1g16080 are shown.
Line ID 155B11
Locus At1g16080 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5514697
Primers used for PCR 17xy - 8474, show primer details
   
Sequence Name 85-K119577-0022-155-B11C-17xy
GenBank Accession LN514596 [GenBank]
Sequencing primer gene specific primer, 17xy: 5'-TTTTTCTACTTTCTACGGACCCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>85-K119577-0022-155-B11C-17xy
TGATTATATTTTGTCGTTTTCCTTTGTCGGGAAACTGAAAGGTTGATGATTGGATTGAGG
TTTAGCGGGAACTGTGTTTCAAACACTCAAACACTTAAAGTATCAACAACCTGCCGTTTT
TTATATTTATCAACGTCATGCCGTTTTATCTTCGTGAGTCATCCAAAACCGATAAGCCAG
AGCCTCAAACCATCGAGTGGCTGTTAAACCATACTGTTCAAATTAGTCCAACCAAAACCA
TGAGAGGCCACAACATCGTCGAAGCCCATTATTATCACCATGGCTAAAGCCAGTAGTACT
CTGTTTGCTGTTTCCTGCTCCGCTTCAGCTCGTTTCTCTTTCCTCCGACGCTCTGAATCT
CTTAAACCCTCAGTTTCAAGAGCGAGATTCGCCGTTCCAATGGCTATGGCCGCCGCGTCA
GCTGCCACCGCTAAAAAACTAGCGCCTGCTGTGATTGTGGGTGGCGGAAGAGTCGGAAGG
GCGTTACAGGAAATGGGTAATGGCGAGGATTTGTTGGTGAAGAGAGGAGAAGCAGTTCCG
GTTGATTTTGAAGGACCCATTTTGGTTTGTACAAGAAATGATGGCTATATTCAATTGATA
ATGGCTTAACCGTTGAATTTTTTCCATTGTAAATGGCTTCATGTCCGGGAAATCTACATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATATAGATGTCCG
   
Sequence Name 69-K119576-0022-155-B11C-8409
GenBank Accession LN512294 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K119576-0022-155-B11C-8409
TGGAAAAAATTCAACGGTTAAGCCATTATCAATTGAATATAGCCATCATTTCTTGTACAA
ACCAAAATGGGTCCTTCAAAATCAACCGGAACTGCTTCTCCTCTCTTCACCAACAAATCC
TCGCCATTACCCATTTCCTGTAACGCCCTTCCGACTCTTCCGCCACCCACAATCACAGCA
GGCGCTAGTTTTTTAGCGGTGGCAGCTGACGCGGCGGCCATAGCCATTGGAACGGCGAAT
CTCGCTCTTGAAACTGAGGGTTTAAGAGATTCAGAGCGTCGGAGGAAAGAGAAACGAGCT
GAAGCGGAGCAGGAAACAGCAAACAGAGTACTACTGGCTTTAGCCATGGTGATAATAATG
GGCTTCGACGATGTTGTGGCCTCTCATGGTTTTGGTTGGACTAATTTGAACAGTATGGTT
TAACAGCCACTCGATGGTTTGAGGCTCTGGCTTATCGGTTTTGGATGACTCACGAAGATA
AAACGGCATGACGTTGATAAATATAAAAAACGGCAGGTTGTTGATACTTTAAGTGTTTGA
GTGTTTGAAACACAGTTCCCGCTAAACCTCAATCCAATCATCAACCTTTCAGTTTCCCGA
CAAAGGAAAACGACAAAATATTAATCAAAATCCCACTATCGTGTATTGACCGTTGGGTCC
GTAGAAGTTAGAAA

Last Updated on 06.12.2018 16:11