Sequences from confirmation amplicon


Confirmation sequences for line 664F02 and hit At1g18890 are shown.
Line ID 664F02
Locus At1g18890 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:6526009
Primers used for PCR TR03 - 8474, show primer details
   
Sequence Name 47-K025447-022-664-F02C-8409
GenBank Accession LN506073 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K025447-022-664-F02C-8409
CCGGACATGAAGCCATTTACAATTGAATATATCCTGTATATTACTATACACAAAAATCAA
AGACTCCCTTTTTTTTAATTGAATCAAACAAAATCCGAAATTTCGAAAGGTCCGACTTTT
CCTCACCTGAACCCGCGTTTTCTCTCCCCCTCCCAACCCAACTCTCCAATTCGCCGGAAA
ATCACCGCCACCGTCACTTCCGGCGTCCATATGAACTAAAGAGATAAGCTTTTTTCTTCT
ATAATCCTTCTTCTTCTTCTTCCTTGATCTCCTTTCTCTGAGTTTGCTGAGTTTCTTAAC
TCAGCCATGGGTAACTGTAACGCCTGTGTAAGGCCAGACTCAAAAGAATCAAAACCATCT
TCAAAGCCGAAAAAACCCAATCGAGATCGGAAATTAAACCCATTCGCCGGAGATTTCACC
AGATCCCCAGCTCCAATACGTGTTCTCAAAGATGTAATCCCTATGAGCAATCAAACTCAG
ATCAGCGACAAATACATCTTAGGTCGTGAATTAGGTCGAGGCGAATTCGGAATCACTTAC
CTCTGTACTGATCGTGAAACCCACGAAGCTTTAGCTTGCAAATCGATTTCAAAGCGAAAG
CTTCGAACAGCTGTCGATATCGAAGACGTTCGTCGTGAGGTAGCGATTATGTCTACTTTA
CCTGAGCATCCAAACGTAGTTAAGCTTAAGGCTAGTTATGAGGATAACGAGAACGTGCAT
CTGGTTATGGAGCTTTGTGAAGGAGGTGAGCTTTTTGATCGGATTGTTGCTAGAGGACAT
TACACGGAGCGTGCTGCTGCAGCTGTTGC
   
Sequence Name 48-K025447-022-664-F02C-TR03
GenBank Accession LN506392 [GenBank]
Sequencing primer gene specific primer, TR03: 5'-AGAGTGACACATCATCACAACCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K025447-022-664-F02C-TR03
CAATCGTTCTCGCAACAGCTGCAGCAGCACGCTCCGTGTAATGTCCTCTAGCAACAATCC
GATCAAAAAGCTCACCTCCTTCACAAAGCTCCATAACCAGATGCACGTTCTCGTTATCCT
CATAACTAGCCTTAAGCTTAACTACGTTTGGATGCTCAGGTAAAGTAGACATAATCGCTA
CCTCACGACGAACGTCTTCGATATCGACAGCTGTTCGAAGCTTTCGCTTTGAAATCGATT
TGCAAGCTAAAGCTTCGTGGGTTTCACGATCAGTACAGAGGTAAGTGATTCCGAATTCGC
CTCGACCTAATTCACGACCTAAGATGTATTTGTCGCTGATCTGAGTTTGATTGCTCATAG
GGATTACATCTTTGAGAACACGTATTGGAGCTGGGGATCTGGTGAAATCTCCGGCGAATG
GGTTTAATTTCCGATCTCGATTGGGTTTTTTCGGCTTTGAAGATGGTTTTGATTCTTTTG
AGTCTGGCCTTACACAGGCGTTACAGTTACCCATGGCTGAGTTAAGAAACTCAGCAAACT
CAGAGAAAGGAGATCAAGGAAGAAGAAGAAGAAGGATTATAGAAGAAAAAAGCTTATCTC
TTTAGTTCATATGGACGCCGGAAGTGACGGTGGCGGTGATTTTCCGGCGAATTGGAGAGT
TGGGTT

Last Updated on 06.12.2018 16:11