Sequences from confirmation amplicon


Confirmation sequences for line 570E08 and hit At1g19340 are shown.
Line ID 570E08
Locus At1g19340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:6686404
Primers used for PCR 17nz - 8474, show primer details
   
Sequence Name 88-K114716-0022-570-E08C-17nz
GenBank Accession LN514888 [GenBank]
Sequencing primer gene specific primer, 17nz: 5'-CCAATGTTTCTTGGTTCTTTGTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>88-K114716-0022-570-E08C-17nz
TTCTATCTCTTCCGATCAAGCAGCTTGCTCATGCTGAAGGAGCTCTCGTGGCTTTGTGGG
TGACAAATAGAGAGAAATTACTAAGTTTTGTTGAGAAAGAGCTATTCCCTGCGTGGGGAA
TTAAGTATGTAGCTACAATGTATTGGTTAAAGGTGAGCTCATCTTGCAGCTCTCAGTCCC
CATCTTGGTTTCTGAGTTGCACATATGACTTGACATTTTACATACTTGCTATGTAAATGT
TTCAGGTGAAACCGGATGGTACACTGATTTGCGACCTGGACCTGGTCCATCATAAACCTT
ATGAGTACCTTCTACTAGGCTACCATTTTACCGAAGTAAGTCAATTCATTTTTTTGTCAC
TTGAATAATCTGAGAAAGGAACTTCTGTTTGAACAGCCATGTTGTGTATACATGTAAATG
GCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAAATGTCCG
   
Sequence Name 40-K114715-0022-570-E08C-8409
GenBank Accession LN503801 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K114715-0022-570-E08C-8409
ACAACATGGGCTGTTCAAACAGAAGTTCCTTTCTCAGATTATTCAAGTGACAAAAAAATG
AATTGACTTACTTCGGTAAAATGGTAGCCTAGTAGAAGGTACTCATAAGGTTTATGATGG
ACCAGGTCCAGGTCGCAAATCAGTGTACCATCCGGTTTCACCTGAAACATTTACATAGCA
AGTATGTAAAATGTCAAGTCATATGTGCAACTCAGAAACCAAGATGGGGACTGAGAGCTG
CAAGATGAGCTCACCTTTAACCAATACATTGTAGCTACATACTTAATTCCCCACGCAGGG
AATAGCTCTTTCTCAACAAAACTTAGTAATTTCTCTCTATTTGTCACCCACAAAGCCACG
AGAGCTCCTTCAGCATGAGCAAGCTGCTTGATCGGAAGAGATAAGAAGTATTGGTTCGGT
AAAGTAGGATACCTAAAACAAAGAACCAAAAAACATTG

Last Updated on 06.12.2018 16:11