Sequences from confirmation amplicon


Confirmation sequences for line 076C12 and hit At1g22400 are shown.
Line ID 076C12
Locus At1g22400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:7906106
Primers used for PCR JA25 - 8474, show primer details
   
Sequence Name 04-K100937-0022-076-C12C-8409
GenBank Accession LN487841 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K100937-0022-076-C12C-8409
AGCCCTTCTCGATGAGAGATAAAAGTGTAGATAAGCCAGGAACGCACAGCCACTGGTTGT
CCAAAAAAGAACCTCCGGGACTCCAAGCTCCTCCGCAACATCAAGAGTAAAGCTCATACA
ACCGTCAGATACAATACAGCTTACCGGAGGAACATTATCTCCAGCGTTGATCCGCTGGAG
AAGCTCTCTGAACGGAGCGAGACAGTTCTTCATGGTGGACTCGCAAAGAGCTGTGATGTC
CTGCGTGGCATCCATGTCTGTCTCTGGTAG
   
Sequence Name 04-K100943-0022-076-C12C-JA25
GenBank Accession LN487842 [GenBank]
Sequencing primer gene specific primer, JA25: 5'-CATGTAGTTTGTGTTCCATATCCG-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K100943-0022-076-C12C-JA25
GCTAAACTCCTCCCGCCAGAGGCTTCTCGTCACCTTCGTCAACACCGTCTACACCACAAT
CGTTTCCTTCGTTCTCGTGGGTCCAATGCCCTAGATGGACTTCCTTCGTTCCGATTTGAG
TCCATTGCTGACGGTCTACCAGAGACAGACATGGATGCCACGCAGGACATCACAGCTCTN
TGCGAGTCCACCATGAAGAACTGTCTCGCTCCGTTCAGAGAGCTTCTCCCGCGGGAT

Last Updated on 06.12.2018 16:11