Sequences from confirmation amplicon


Confirmation sequences for line 654D08 and hit At1g23680 are shown.
Line ID 654D08
Locus At1g23680 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:8377036
Primers used for PCR CW78 - 8474, show primer details
   
Sequence Name 01-K104151-0022-654-D08C-CW78
GenBank Accession LN486552 [GenBank]
Sequencing primer gene specific primer, CW78: 5'-AGAAGAAGAATTCGGATATGGGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K104151-0022-654-D08C-CW78
GATATAGAGAAGTTTCTTACCGTAAGATCTTTTTGGTTGTCTTCGACAATTAGACTTGTT
GGGAGAGCTCCAGAAAACAAAAGGAATTTCCAGGATACAACTTTCTCTACCTCCGCAATC
CGCCTCGGTCCATCCTTCATCAAAAGTTTTCTTGATTTGCTTTCCTTCATAAGAAAAATC
ACACTCTTAAGTACTTGCAATATTGTATTCTTCTTGACTCTTCGGCTCCATTTGCTTGCA
CAAACGTTTTGAATATATGTATAGTCGCTCCAGGATATATTCAATTGTAAATGGCTTCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 01-K104152-0022-654-D08C-8409
GenBank Accession LN486553 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K104152-0022-654-D08C-8409
TCGGACTGAGCCATTTACATTGAATAATCCTGGAGCGACTATACATATATTCAAAACGTT
TGTGCAAGCAAATGGAGCCGAAGAGTCAAGAAGAATACAATATTGCAAGTACTTAAGAGT
GTGATTTTTCTTATGAAGGAAAGCAAATCAAGAAAACTTTTGATGAAGGATGGACCGAGG
CGGATTGCGGAGGTAGAGAAAGTTGTATCCTGGAAATTCCTTTTGTTTTCTGGAGCTCTC
CCAACAAGTCTAATTGTCGAAGACAACCAAAAAGATCTTACGGTAAGAAACTTCTCTATA
TCCCTCTTAATGATTTAAAAGATGATATCCCATATCCGAATTCTTCTT

Last Updated on 06.12.2018 16:11