Sequences from confirmation amplicon


Confirmation sequences for line 382A12 and hit At1g24793 are shown.
Line ID 382A12
Locus At1g24793 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:8767662
Primers used for PCR 56wj - 8474, show primer details
   
Sequence Name 34-K118380-0022-382-A12C-56wj
GenBank Accession LN501594 [GenBank]
Sequencing primer gene specific primer, 56wj: 5'-TTCAGGTAACAAACCTCGACTTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K118380-0022-382-A12C-56wj
CAATATCAACTTCCTTCGCCTTAGAGCTGATAACAAACGCTCAACGGTTCGGATTTTGAG
CTCATCTTTCAACAGCGTCGTGCAAAGAGGCGATTCCTGAGCAAATTCAATCGACGCAGG
GATGAATCTGGATCGAAACTCGAAGAACCTACCAGCTCCGGCGATCTCCGGATTTAGCTT
CACCGTCGAGAACTTACCGGAGTGCAGAGATTTTCCTTTCACTTCCACAGATCCTGCGAG
AGTTTGCTGTAATCGTCCACTCTACAGTATTGAACCCCAAGACAAAAAAAACACACTCAA
AATGATTCCAATGGATAATAAAGTCGCATATACATGTAAGCGTGTGCGGGAGAGAGGTTT
ACTGGATTCAAGGAGACTGTCGGCGACGACGCGGCGTATGAGTATTAGCAATCTACATGG
ATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTT
TCAATTCAAAAATGTAGATTCC
   
Sequence Name 24-K118379-0022-382-A12C-8409
GenBank Accession LN497187 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K118379-0022-382-A12C-8409
CCGACAGTCTCCTTGAATCCAGTAAACCTCTCTCCCGCACACGCTTACATGTATATGCGA
CTTTATTATCCATTGGAATCATTTTGAGTGTGTTTTTTTTGTCTTGGGGTTCAATACTGT
AGAGTGGACGATTACAGCAAACTCTCGCAGGATCTGTGGAAGTGAAAGGAAAATCTCTGC
ACTCCGGTAAGTTCTCGACGGTGAAGCTAAATCCGGAGATCGCCGGAGCTGGTAGGTTCT
TCGAGTTTCGATCCAGATTCATCCCTGCGTCGATTGAATTTGCTCAGGAATCGCCTCTTT
GCACGACGCTGTTGAAAGATGAGCTCAAAATCCGAACCGTTGAGCATTTGTTATCAGCTC
TTGAGGCGAAGGGAGTTGATAATTGCAGGATTCAAATCGAGAGCGAAAGTTCCGATGATC
GAGAAGTCGAGG

Last Updated on 06.12.2018 16:11