Sequences from confirmation amplicon


Confirmation sequences for line 088B03 and hit At1g25280 are shown.
Line ID 088B03
Locus At1g25280 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:8866770
Primers used for PCR IH33 - 8474, show primer details
   
Sequence Name 59-K100853-0022-088-B03C-8409
GenBank Accession LN509935 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K100853-0022-088-B03C-8409
GCCATTTACAACAATTGAATAGAGAAGAGGATGTTAATAGTAGACGTCTGACTCTCACAG
TACAGCAACTGAGTATTGAGACAAATTTTGATTTGCCTAAAAAAAACCAAAAGCATAGAT
AAAAGAGAGAGAGAGACAAGGGATTAGCATGAATGATCAAGGAGACGAGTGGTTTCTTCT
ATTCACAAGCAAGCTTGGTGTCAAAGCTGCTTAAGCATATCGCAAACGCCTGAAACGCCG
ATAATGGATACCTATAGTCCATTGTGAACATATCTTTCCCTACTTTACCAAACTGGAGAA
TCACCTTGTCTTGCTCAGGGTGAGCAGGTGCACTTGTTGGTGCTGCTGCTGCACCTGTCC
CTTGAGGCTGCGGCTGTCTTGCTGCTACAAGCTGGAAATTCTTAACCGATGCAACTGTCA
CACGTCCGCGGAAATTCAAACACCAGCATTGCAACTGCTCGTGCCACCTTGGCTGCTTGT
TCTTTAGAATCAACGGTCTGAAACTCGCTTCTTCTTCGTTGTCGTCGCAGGATATTCCCA
TTTCGGAGAATCTAGAACTGCTGAAATCGAGGGAGCGGTGGTCAAATGATGATTTGGAGA
AGGAGATGTTACTGCGGAATGAGTCGT
   
Sequence Name 59-K100859-0022-088-B03C-IH33
GenBank Accession LN509936 [GenBank]
Sequencing primer gene specific primer, IH33: 5'-ACCATACTCTCTCGACGACTCATT-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K100859-0022-088-B03C-IH33
TCATTTGACCCCGCTCCCTCGATTTCGCAGTTCTAGATTCTCCGAAATGGGAATATCCTG
CGACGACAACGAAGAAGAAGCGAGTTTCAGACCGTTGATTCTAAAGAACAAGCAGCCAAG
GTGGCACGAGCAGTTGCAATGCTGGTGTTTGAATTTCCGCGGACGTGTGACAGTTGCATC
GGTTAAGAATTTCCAGCTTGTAGCAGCAAGACAGCCGCAGCCTCAAGGGACAGGTGCAGC
AGCAGCACCAACAAGTGCACCTGCTCACCCTGAGCAAGACAAGGTGATTCTCCAGTTTGG
TAAAGTAGGGAAAGATATGTTCACAATGGACTATAGGTATCCATTATCGGCGTTTCAGGC
GTTTGCGATATGCTTAAGCAGCTTTGACACCAAGCTTGCTTGTGAATAGAAGAAACCACT
CGTCTCCTTGATCATTCATGCTAATCCCTTGTCTCTCTCTCTCTTTTATCTATGCTTTTG
GTTTTTTTTAGGCAAATCAAAATTTGTCTCAATACTCAGTTGCTGTACTGTGAGAGTCAG
ACGTCTACTATTAACATCCTCTTCTCTATTCAATTGTTGTTAAATGGCTTCATGTCCGGG
AAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATT
ACCAATTTTTTTTCAATTCAAAAATGTAGATGTC

Last Updated on 06.12.2018 16:11