Sequences from confirmation amplicon


Confirmation sequences for line 082G04 and hit At1g25360 are shown.
Line ID 082G04
Locus At1g25360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:8894975
Primers used for PCR 02ny - 3144, show primer details
   
Sequence Name 31-K114678-0022-082-G04C-02ny
GenBank Accession LN500274 [GenBank]
Sequencing primer gene specific primer, 02ny: 5'-CCGTCCATGGAAATATCATTACTT-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K114678-0022-082-G04C-02ny
TTGATGTTTATTGTAAATCGTCAGAGCTAAATTATGCACGCCAACTGTTCGATGAAATCT
CTGAACCAGATAAAATTGCTAGAACCACCATGGTCTCTGGCTATTGTGCTTCCGGTGATA
TCACCCTCGCTCGAGGGGTTTTTGAGAAAGCTCCTGTGTGTATGCGTGATACTGTAATGT
ACAATGCGATGATCACTGGCTTTTCACATAACAATGATGGTTACTCTGCTATTAACCTCT
TTTGTAAGATGAAGCATGAAGGATTCAAACCTGACAACTTCACTTTCGCAAGTGTTCTCG
CGGGTTTAGCGTTAGTTGCCGATGACGAGAAGCAATGTGTGCAATTTCATGCCGCAGCTC
TGAAATCTGGTGCTGGTTATATGGATCAATTGTAAATGACAATCTGATCCCCGGGTACCC
TGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTG
GGATTGTGCGTCATCCCTTACGTCAGTGGAGATAT
   
Sequence Name 07-K114677-0022-082-G04C-3144
GenBank Accession LN489383 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K114677-0022-082-G04C-3144
CTTCGCAGACCCTTCCTCTATTAAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCCG
GGGATCAGATTGTCATTTACAATTGATCCATATAACCAGCACCAGATTTCAGAGCTGCGG
CATGAAATTGCACACATTGCTTCTCGTCATCGGCAACTAACGCTAAACCCGCGAGAACAC
TTGCGAAAGTGAAGTTGTCAGGTTTGAATCCTTCATGCTTCATCTTACAAAAGAGGTTAA
TAGCAGAGTAACCATCATTGTTATGTGAAAAGCCAGTGATCATCGCATTGTACATTACAG
TATCACGCATACACACAGGAGCTTTCTCAAAAACCCCTCGAGCGAGGGTGATATCACCGG
AAGCACAATAGCCAGAGACCATGGTGGTTCTAGCAATTTTATCTGGTTCAGAGATTTCAT
CGAACAGTTGGCGTGCATAATTTAGCTCTGACGATTTACAATAAACATCAATCAAACGGT
TCAGGATATGGGCACGTGGCTGAAACCCGAAAGTAAT

Last Updated on 06.12.2018 16:11