Sequences from confirmation amplicon


Confirmation sequences for line 199B12 and hit At1g25360 are shown.
Line ID 199B12
Locus At1g25360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:8894399
Primers used for PCR 06nz - 8474, show primer details
   
Sequence Name 46-K114664-0022-199-B12C-06nz
GenBank Accession LN505944 [GenBank]
Sequencing primer gene specific primer, 06nz: 5'-CCATGGTGGTTCTAGCAATTTTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K114664-0022-199-B12C-06nz
TTTAGCTCTGACGATTTACAATAAACATCAATCAAACGGCCCTCTATATGGGCACGTGGC
TGAAACCCGAAAGTAATGATATCTCCATGGACGGCTCGCGCGAGCTGCAGAGATGTTCGT
CGGAGCGGCAAACAGAGGCGGAGATTCGCGGCGTAGCGGTTGGCGATGGCTCGGACAAGG
TCTGGATTCGGTTGCATTTCATGTTTATGGTTCGGTTCGAAATTTTCAAACTGCAATGAG
TATCTACATGTAAATGGCTGCAATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGGTTATTA
   
Sequence Name 17-K114663-0022-199-B12C-8409
GenBank Accession LN493958 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K114663-0022-199-B12C-8409
TTGAAATATATTGCAGCCATTTACATGTAGATACTCATTGCACTTATAAAATTTCGAACC
GAACCATAAACATGAAATGCAACCCATTCCAGACCTTGTCCGAGCCATCGCCAACCGCTA
CGCCGCGAATCTCCGCCTCTGTTTGCCGCTCCGACGAACATCTCTGCAGCTCGCGCGAGC
CGTCCATGGAAATATCATTACTTTCGGGTTTCAGCCACGTGCCCATATCCTGAACCGTTT
GATTGATGTTTATTGTAAATCGTCAGAGCTAAATTATGCACGCCAACTGTTCGATGAAAT
CTCTGAACCAGATAAAATTGCTAGAACCACCATGG

Last Updated on 06.12.2018 16:11