Sequences from confirmation amplicon


Confirmation sequences for line 016F07 and hit At1g28690 are shown.
Line ID 016F07
Locus At1g28690 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:10081802
Primers used for PCR 22mk - 8474, show primer details
   
Sequence Name 28-K114225-0022-016-F07C-22mk
GenBank Accession LN498947 [GenBank]
Sequencing primer gene specific primer, 22mk: 5'-GTTTCAACGGGAATGTTGAGTAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K114225-0022-016-F07C-22mk
TGAGATTCTCATGCTTACATTAGCTGTGTGAATTGTGTGAATAAAGATTGAAACTTTACG
CGTAATTTACAGAGGCTCTGTTTCGAACTTTCCAAAATGCAAAGAACGAGAGAGAGAGAA
GTCGGTCAAACGACAGCGTTTGATGTATTTGATAAAACGACAGCGTTTGATGTATTTGAT
AAAACGACAGCGTTTGATATAGTTGATATATAATGTGTTTCCCGGTACCGTGAACGTCGG
CTCGATTGTACCTGCGTTCAAATACTTTGCGATCGTGTTGCGCGCCTGCCCGGTGCGTCG
GCTGATCTCACGGATCGACTGCTTCTCTCGCAACGCCATCCGACGGATGATGTTTAAAAG
TCCCATGTGGATCACTCCGTTGCCCCGTCGCTCACCGTGTTGGGGGGAAGGTGCACATGG
CTCAGTTCTCAATGGAAATTATCTGCCTAACCGGCTCAGTTCTGCGTAGAAACCAACATG
CAAGCTCCACCGGGTGCAAAGCGGCAGCGGCGGCAGGATATATTCAATTGTAAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 08-K114224-0022-016-F07C-8409
GenBank Accession LN489822 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K114224-0022-016-F07C-8409
ATTTCATTGAATATATCCTGCCGCCGCTGCCGCTTTGCACCCGGTGGAGCTTGCATGTTG
GTTTCTACGCAGAACTGAGCCGGTTAGGCAGATAATTTCCATTGAGAACTGAGCCATGTG
CACCTTCCCCCCAACACGGTGAGCGACGGGGCAACGGAGTGATCCACATGGGACTTTTAA
ACATCATCCGTCGGATGGCGTTGCGAGAGAAGCAGTCGATCCGTGAGATCAGCCGACGCA
CCGGGCAGGCGCGCAACACGATCGCAAAGTATTTGAACGCAGGTACAATCGAGCCGACGT
TCACGGTACCGGGAAACACATTATATATCAACTATATCAAACGCTGTCGTTTTATCAAAT
ACATCAAACGCTGTCGTTTTATCAAATACATCAAACGCTGTCGTTTGACCGACTTCTCTC
TCTCTCGTTCTTTGCATTTTGGAAAGTTCGAAACAGAGCCTCTGTAAATTACGCGTAAAG
TTTCAATCTTTATTCACACAATTCACACAGCTNATGTAAGCATGAGAATCTTCAGATTCA
CGTCAATCTCACCGCGGATTTTGCCGTCGAATCATTACTCAA

Last Updated on 06.12.2018 16:11