Sequences from confirmation amplicon


Confirmation sequences for line 387B10 and hit At1g29280 are shown.
Line ID 387B10
Locus At1g29280 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:10236964
Primers used for PCR NT42 - 8474, show primer details
   
Sequence Name 64-K036524-022-387-B10C-NT42
GenBank Accession LN511142 [GenBank]
Sequencing primer gene specific primer, NT42: 5'-TTGAGAAACTAAAACAAAAACGCC-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K036524-022-387-B10C-NT42
TGAGCGACATAATATCCATAAAATATCACCACGTGTCAAAAGATCTCAACTTGTGACCCC
ACTACGCTACTCCGGTGACGAAACACCACAGAACACTCCGGCAACTCGCCGAGATCGGCG
AACAAAGACTCATCCTCTTCTCCCATCGGAAAGAACACCGCCACGTCATCAGCCCCCGAG
ACAGCCGTCTTTTTCTCCGACGAGAAAATCGGGCTCTCAAGAATCGTCGAAGATGTTGTC
TCCATCTCCGTAAACCATGCGAACTCGTCGACACATGATGGCGTCGTTTCAATCCCCCTT
CCAAGAACCATAAACTTATTATCCTCCTCCTCCGCCTCTGGCTCTACCTCAGGCTCAGGT
TCGGGTTTGGGCTCGGGTTTAGGTTTAGGCCCGTTTCTTGTGGAAGAAGTGAGAGGCCAA
GGATGGTTATGCTCAGAAGTGTAAGTGATGAGAATCATCGTTGGATCATCTCTGCTTCTC
TCGACTTGCTTCCTCGCCGGACAACCTTTTGTGCTACTACAACGGTAATAACCTCTAGGC
TCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
CCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCG
TATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTG
TCTACA
   
Sequence Name 40-K036524-022-387-B10C-8409
GenBank Accession LN503629 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K036524-022-387-B10C-8409
GCCATTTACAATTGAGCCTAGAGGTTATTACCGTTGTAGTAGCACAAAAGGTTGTCCGGC
GAGGAAGCAAGTCGAGAGAAGCAGAGATGATCCAACGATGATTCTCATCACTTACACTTC
TGAGCATAACCATCCTTGGCCTCTCACTTCTTCCACAAGAAACGGGCCTAAACCTAAACC
CGAGCCCAAACCCGAACCTGAGCCTGAGGTAGAGCCAGAGGCGGAGGAGGAGGATAATAA
GTTTATGGTTCTTGGAAGGGGGATTGAAACGACGCCATCATGTGTCGACGAGTTCGCATG
GTTTACGGAGATGGAGACAACATCTTCGACGATTCTTGAGAGCCCGATTTTCTCGTCGGA
GAAAAAGACGGCTGTCTCGGGGGCTGATGACGTGGCGGTGTTCTTTCCGATGGGAGAAGA
GGATGAGTCTTTGTTCGCCGATCTCGGCGAGTTGCCGGAGTGTTCTGTGGTGTTTCGTCA
CCGGAGTAGCGTAGTGGGGTCACAAGTTGAGATCTTTTGACACGTGGTGATATTTTATTG
GATATTATTGTCGCTCACATGGGAAGCGTTGTACGCACGGCGTTTTTGTTTAGTT

Last Updated on 06.12.2018 16:11