Sequences from confirmation amplicon


Confirmation sequences for line 228C09 and hit At1g29690 are shown.
Line ID 228C09
Locus At1g29690 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:10379641
Primers used for PCR 54xo - 8474, show primer details
   
Sequence Name 51-K119542-0022-228-C09C-54xo
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 54xo: 5'-GGCACTCATATTGTAACTTCGGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K119542-0022-228-C09C-54xo
CAGACACATCAGTCTTCTCCTTTACAGTATCTGAAATAGAAAACTATGTGAATGACATGA
TAAAGCACAGATTTCATGAAGCAGAGAGTCAATCTATTACCGGCCCCTTGAAATACAAAG
ACAAGGTTAGTACAACGTTAAGGGCATGGCGAGATTACTCTTGTTAATGAATTTTATTTC
CTATAAAATTATGGTTTGATAAAGATATTAGTTGAAAAAGCTCTCTCCTGTAGGATATCA
CAGTAATATTTAGGCGAAGAGGAGGCGACGATCTTGAGCAAAGCCATGCTCGTTGGGCTG
AGACTGTACCCGCAGCTCCAGACATTATCAACATGACTTTTACTCCTATTGTTTCTCTCC
TTGAAGGTGTGCCTGGTTTACGGCATTTGACTCGTGCTATCGAACTTTACTTGGAATGTG
AGTTTGTATCTAAAAATTTAACTTCTTTGTATTTGAATCCACCTGGTTACATTCTCATAG
AAATTTCTCGATATTTTTACCAGATAAGCCTCCTATTGAAGATTTACAATACTTCTTGGA
TTACCAAATAGCTCGAGCTTGGGCTCCCGAACAGAGTAACCTCCAACGTAAAGAACCTGT
GTGTTCTTCTCTTCAATTCAGCTTAATGGGTCCCAAACTATTCATCAGCGCTGATCAGGT
AACATAGAGCGGTCGATTCCTTTGTTTAGTTACTGTTCTGTTTATGTATGAACTGAACAT
ATTTGCCTTTTGGGTAATTTCAGGTAACTGTTGGGCGTAAGCCGGTCACAGGTCTCAGAC
TGAGCTTAGAAGGAAGCAAACAAACCGTCTCTCTATCAATTTACAACACTTAGTCTCTCT
CCCTAAATCCTGCAGCCACATTGGGACTCTCACGTGCCAATAGGTGCAC
   
Sequence Name 19-K119541-0022-228-C09C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K119541-0022-228-C09C-8409
CATTTCATTGAATTATCCTGTTGCTCGCAATGATAACAACAATGAGTAAATGGCTCCAGT
GACAATGTGAACGCCGGAGAGATCGCCGATGTGGGTCTCTGTGTGTTCGATAGGTGAGGT
GCTTACGTGAGAGAAGTTCTTCCATTTGATTGGCTCGAACCAGCGGCTATCTTGCTCCTC
TGGTCCTTGCCATTTCGGTGCACCTATTGGCACGTGAGAGTCCCAATGTGGCTGCAGGAT
TTTAGGGAGAGAGACTAAGTGTTGTAAATGGATAGAGAGACGGTTTTGTTTGCTTCCTTC
TAAGCTCAGTCTGAGACCTGTGACCGGCTTACGCCCAACAGTTACCTGAAATTACCCAAA
AGGCAAATATGTTCAGTTCATACATAAACAGAACAGTAACTAAACAAAGGAATCGACCGC
TCTATGTTACCTGATCAGCGCTGATGAATAGTTTGGGACCCATTAAGCTGAATTGAAGAG
AAGAACACACAGGTTCTTTACGTTGGAGGTTACTCTGTTCGGGAGCCCAAGCTCGAGCTA
TTTGGTAATCCAAGAAGTATTGTAAATCTTCAATAGGAGGCTTATCTGGTAAAAATATCG
AGAAATTTCTATGAGAATGTAACCAGGTGGATTCAAATACAAAGAAGTTAAATTTTTAGA
TACAAACTCACATTCCAAGTAAAGTTCGATAGCACGAGTCAAATGCCGTAAACCAGGCAC
ACCTTCAAGGAGAGAAACAATAGGAGTAAAAGTCATGTTGATAATGTCTGGAGCTGCGGG
TACAGTCTCAGCCCAACGAGCATGGCTTTGCTCAAGATCGTCGCCTCCTCTTCGCCTAAA
TATAACTGTGATATCCTACAGGAGAGAGCTTTTTCAACTAATATCTTTATCAAACCCTAA
T

Last Updated on 06.12.2018 16:11