Sequences from confirmation amplicon


Confirmation sequences for line 477H04 and hit At1g30460 are shown.
Line ID 477H04
Locus At1g30460 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:10773891
Primers used for PCR AC42 - 8474, show primer details
   
Sequence Name 07-K027916-022-477-H04C-8409
GenBank Accession LN489125 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K027916-022-477-H04C-8409
CATTTACATTGAATATTAGAGGACAGGGTTACCCGGGGACCATCTTTATGGCGCCTAAGA
GTCATGTTTTCTTTATTTCAGATTGGTCACCAAGAGTTGATTGCCTATTCTTGTGCTCTA
GTGTTGCTATTTTGCCTATAACGTAATTTTATTCAGATATTAGTAAGAGATTGTGTAATT
CATGTAGCTATATCTTCTAAAATCCCATACAATTGATCTAAAATGAGTGTGTGAAGAAGA
ATGGGATTCTTCAAACGTTGTCTAGTATTTCGGCTTTCGAAGATGCTCATCTTGACTGCT
CGATATTAAGTTTGATGTAACTCTTGTATGTTTGATTTTATGGATTCGTTAACTGTATAT
ATACCTTGTGGTATATTATTACTCTTCATAGGTAATCCCTTACATAATTTAGGATCTTGT
TTTTTTCAGGTATTTTGTAGTTAAAAGTAACAATCGAGAAAATTTTGAATT
   
Sequence Name 08-K027916-022-477-H04C-AC42
GenBank Accession LN489588 [GenBank]
Sequencing primer gene specific primer, AC42: 5'-CCTTGTTGCACAGATAATTCAAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K027916-022-477-H04C-AS42
ACTACAAATACCTGAAAAACAAGATCCTAAATTATGTAAGGGATTACCTATGAAGAGTAA
TAATATACCACAAGGTATATATACAGTTAACGAATCCATAAAATCAAACATACAAGAGTT
ACATCAAACTTAATATCGAGCAGTCAAGATGAGCATCTTCGAAAGCCGAAATACTAGACA
ACGTTTGAAGAATCCCATTCTTCTTCACACACTCATTTTAGATCAATTGTATGGGATTTT
AGAAGATATAGCTACATGAATTACACAATCTCTTACTAATATCTGAATAAAATTACGTTA
TAGGCAAAATAGCAACACTAGAGCACAAGAATAGGCAATCAACTCTTGGTGACCAATCTG
AAATAAAGAAAACATGACTCTTAGGCGCCATGTATGATGGTCCCCGGGTAACCCTGTCCT
CTATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGT
ATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAAT
GTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACG
ATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATT
TCGACGTGTC

Last Updated on 06.12.2018 16:11