Sequences from confirmation amplicon


Confirmation sequences for line 053D01 and hit At1g30680 are shown.
Line ID 053D01
Locus At1g30680 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:10882719
Primers used for PCR PF69 - 3144, show primer details
   
Sequence Name 25-K102867-0022-053-D01C-3144
GenBank Accession LN497392 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K102867-0022-053-D01C-3144
TCCACTATCCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGAC
AGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGAT
CCTATAGAGAAGGTTGAAAGAAAAATTACGGTGGAGGGTATAGAGCTAGAACCTCTCTGT
GATGAGGTTGTTGTCTTCTTTCATATGTTTCTTTTGACATACTTATTCTTATTATTTCTT
CTGTGTCTACTGCTTTCCTTAGATGATGACTGAACTTTGTGAATTCTTCTTTTCGTTGTA
GTTATATGAGTTTGATAAATTTTATTTGTGTTCTTGGTTACGTTTCAGATTCAAGATTAT
TTCGCTGCAAGAGCGATTTCACGGAAAACACTCGAGAGAAATCGGGTTATGCAGAAAAGA
ATAGGTGACGAGTAGNCTTACGA
   
Sequence Name 25-K102868-0022-053-D01C-PF69
GenBank Accession LN497393 [GenBank]
Sequencing primer gene specific primer, PF69: 5'-TCGTAAGCTACCTCGTCACCTATT-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K102868-0022-053-D01C-PF69
TTCCGTGAATCGCTCTTGCAGCGAAATAATCTTGAATCTGCCACGATAGCCAAGAACACA
AATAAAATTTATCAAACTCATATAACTACAACGAAAAGAAGAATTCACAAAGTTCAGTCA
TCATCTAAGGAAAGCAGTAGACACAGAAGAAATAATAAGAATAAGTATGTCAAAAGAAAC
ATATGAAAGAAGACAACAACCTCATCACAGAGAGGTTCTAGCTCTATACCCTCCACCGTA
ATTTTTCTTTCAACCTTCTCTATAGGATCAAACACTGATAGTTTAAACCGAAGGCGGGAA
ACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAG
AGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATAT
CACATCAATCCACTTGCTTTGAAGACGTGGTTGGAACGTCTTCTTTTTCCACGATGCTCC
TCGTGGGTGGGGGTCCAACTTTGGG
Primers used for PCR PF68 - 8474, show primer details
   
Sequence Name 46-K118051-0022-053-D01C-PF68
GenBank Accession LN506001 [GenBank]
Sequencing primer gene specific primer, PF68: 5'-CTTGATATGTCCGACGGTATTTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K118051-0022-053-D01C-PF68
TTGAGTATCTTGAAAAAGGCTAATTTAGATTGTCCACTGTTCTGGTGGGTACATCAGTGT
GAAGGTGGAAACTCTGGAGAAAAAAGCTTGTCTCTTTTTATAGCCCCTGATGGGTATGTT
ATCTTATTATCTCATCAAAATATGTTTTGGTCATGAGACTGTTGTTAATCATGGTGTTTT
ATTTTCTAATTTGTAGTTCATCAGCTACATGGAATTGCTTTAGGGGGAAGTGTGGGTTAA
AAGGTGGAGTTCGGGTGAGTTGCGATGGTTGAGTACGAGTACGAGTACGACCAACATTTT
ATGAAGTTAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGTAGATGTC
   
Sequence Name 10-K118050-0022-053-D01C-8409
GenBank Accession LN490857 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K118050-0022-053-D01C-8409
ATTACATTGAATATATCCTAACTTCATAAAATGTTGGTCGTACTCGTACTCGTACTCAAC
CATCGCAACTCACCCGAACTCCACCTTTTAACCCACACTTCCCCCTAAAGCAATTCCATG
TAGCTGATGAACTACAAATTAGAAAATAAAACACCATGATTAACAACAGTCTCATGACCA
AAACATATTTTGATGAGATAATAAGATAACATACCCATCAGGGGCTATAAAAAGAGACAA
GCTTTTTTCTCCAGAGTTTCCACCTTCACACTGATGTACCCACCAGAACAGTGGACAATC
TAAATTAGCCTTTTTCAAGATACTCAACATGAATCCCAAAAGAAAGAGCAAACATCAATG
AAAGCAACATCCAGATAGTACTTAAGTCTTGGCAAATATAAGCATATGAACAAGACTATA
AGAAATACCGTCGAACTTTATCA

Last Updated on 06.12.2018 16:11