Sequences from confirmation amplicon


Confirmation sequences for line 245A10 and hit At1g31460 are shown.
Line ID 245A10
Locus At1g31460 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:11262445
Primers used for PCR 27xy - 8474, show primer details
   
Sequence Name 32-K119596-0022-245-A10C-27xy
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 27xy: 5'-GTATGAACATATGGGGACTGCTAA-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K119596-0022-245-A10C-27xy
TCGTTCGATTGCTCATAAGTAGATTGGAGTTTTGGTTATACTACATTGCGTATTTCTGTG
GTGGCACAATTGGCCTTGTTTACAGCAACAATTTGGGACAGATTGCTCAGTCTTTGGGAC
AAAACTCTACTACACTAGTCACAATCTACTCTTCATTCTCCTTCTTCGGTAGATTGCTCT
CTGCCGCACCAGATTTTATGCACAAGTAAGTTTCATTTTAGATTAAGGAATGTCTTAATG
TGAAATTGAAATAACATATTCTCATCAAGAATATTGGATCTTGCAGGAGATTTCGTTTAA
CAAGAACCGGCTGGTTTGCAATCGCACTCTTACCAACTCCAATCGCTTTCTTTTTACTAG
CGGTATCATCCTCACAACAAACAGCATTGCAGACCGCAACCGCATTAATCGGTCTGAGTT
CAGGTTTCATATTCGCAGCCGCAGTCTCCATAACATCTGATCTCTTTGGACCCAATAGCG
TAGGTGTTAACCACAACATTCTCATCACAAACATACCAATCGGCTCACTCTTATACGGTT
ACATCGCTGCCTCCATTTACGAAGCCAACGCAAGCCCCGATATAACACCAATAGTGTCTG
ACTCAATCGTTTGCATCGGAAGAGATTGCTACTTCAAAACGTTTGTGTTTTGGGGTTGCT
TGTCTATCCTCGGCGTCGTCTCAAGTCTGTCACTGTACATACGAACCAAACCGGTTTATC
ACCGGCTTGAACAAGACAAGGTTTCATTAACATCATCGTACAAGGATCTTGATCCATTGT
AAATCAAATCTGTAACACAAAGAAACCACAGAAAAGATAATTAGTGGAACTTATTGAGAG
AAAGACACCAGTTTTTGCTTACATATTATATGAATAGAATCTTAAATAAAATTGTCTTTN
ATAAGTAATTAGATTAAAGCTTAATCACACATTTGGTGGGTTAGTCATCAAAATTGACGC
GCAC
   
Sequence Name 08-K119595-0022-245-A10C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K119595-0022-245-A10C-8409
TGAGGCGACGGAAGCGAGATTCGAGATCATCTGGAAGAGTAGAATCGGTGAATCCAGAAC
AATTGATAGCAGCTACTTGCTCCAAATCAGAAAGCTCCTTCGCTTGCGAAATTAGCTCTT
CCACTGCTGAATCGTCTGTATCTGAAAAATACCCAAAATCCGCCATTATCGATGAGATCA
AGCTTCTTAGGGTTTATTTTTTGCTTTTTGGTGGAGACGAAAGAAATAAGAGTGAGAGAG
AAAGAAAATGATAAAAGTCGTCACAACGGCTAATTCATGATTTTGTCAGTTACGTTGGCT
CCGATTCTGTGCGCGTCAATTTTGATGACTAACCCACAAAATGTGTGATTAAGCTTTAAT
CTAATTACTTTATTAAAGACAATTTTAATTTAAGATTCTATTCATATAATATGTAAGCAA
AAACTGGTGTCTTTCTCTCAATAAGTTCCACTAATTATCTTTTCTGTGGTTTCTTTGTGT
TACAGATTTGATTTACAATGGATCAAGATCCTTGTACGATGATGTTAATGAAACCTTGTC
TTGTTCAAGCCGGTGATAAACCGGTTTGGTTCGTATGTACAGTGACAGACTTGAGACGAC
GCCGAGGATAGACAAGCAACCCCAAAACACAAACGTTTTGAAGTAGCAATCTCTTCCGAT
GCAAACGATTGAGTCAGACACTATTGGTGTTATATCGGGGCTTGCGTTGGCTTCGTAAAT
GGAGGCAGCGATGTAACCGTATAAGAGTGAGCCGATTGGTATGTTTGTGATGAGAATGTT
GTGGTTAACACCTACGCTATTGGGTCCAAAGAGATCAGATGTTATGGAGACTGCGGCTGC
GAATATGAAACCTGAACTCAGACCGATTAATGCGGTTGCGGTCTGCAATGCTGTTTGTTG
TGAGGATGATACCGCTAGTAAAAAGAAAACGATTGG

Last Updated on 06.12.2018 16:11