Sequences from confirmation amplicon


Confirmation sequences for line 258F08 and hit At1g31880 are shown.
Line ID 258F08
Locus At1g31880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:11447812
Primers used for PCR CQ02 - 8474, show primer details
   
Sequence Name 09-K105632-0022-258-F08C-CQ02
GenBank Accession LN490175 [GenBank]
Sequencing primer gene specific primer, CQ02: 5'-AGAGAGATTGGTGAAAGATTCCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K105632-0022-258-F08C-CQ02
ATCTCTGTCCTAAACACAGTAACCTCTATATACTAATGACTTCAAACTCTATTCTTTGGT
AAAGAAAACGAATAATAGTCAAATATATGGACCCTTTTTAAGTCCTCTAAGATACGTTAA
TTGGGGTTTGTGTCTTTGTTTGTTTCACGAGCAAGGCAATTAGAGAAAAGCAATAAAATA
AAGTGAAAGAAAATAAAAGCAGAAGAAGGATCAACAAAGAAAGAAGAGAGAGACCTGAAT
GGTTAGGCTTTTGACGGCTTCTTTAGTATTGGGAGTGGTGCCTCCACGCGCTCCATGTTC
GACTTCTTCACCTCCGTCTGTAAATGGCTGCAGGATATATTCAATTGTAAATGGCTTCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 25-K105633-0022-258-F08C-8409
GenBank Accession LN497442 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K105633-0022-258-F08C-8409
ATTTACATTGAATAATCCTGCAGCCATTTACAGACGGAGGTGAAGAAGTCGAACATGGAG
CGCGTGGAGGCACCACTCCCAATACTAAAGAAGCCGTCAAAAGCCTAACCATTCAGGTCT
CTCTCTTCTTTCTTTGTTGATCCTTCTTCTGCTTTTATTTTCTTTCACTTTATTTTATTG
CTTTTCTCTAATTGCCTTGCTCGTGAAACAAACAAAGACACAAACCCCAATTAACGTATC
TTAGAGGACTTAAAAAGGGTCCATATATTTGACTATTATTCGTTTTCTTTACCAAAGAAT
AGAGTTTGAAGTCATTAGTATATAGAGGTTACTGTTGTTTAGGACAGAGATCTTACAATG
AGGTAACTTCACTTCATTTTTTGGAATCTTT
Primers used for PCR CQ03 - 8474, show primer details
   
Sequence Name 10-K105632-0022-258-F08C-CQ03
GenBank Accession LN490653 [GenBank]
Sequencing primer gene specific primer, CQ03: 5'-TATCCGACCATGGTGTAATGTAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K105632-0022-258-F08C-CQ03
GGTTAGTACTTGAAAATTTAGCATTGGTTCTTTATGAGTTAAACTACTAACCAGTCAAGT
TACATTTTCTTTTTGTTAAACATGAGTAGTTGTTTCCTGACTAGAGATATGTCAACAACT
TTGTCCTTTTGTGTTTGTGTTTTGCGTTATAAGAAAACGACAATTGAAATCCAAATACCC
TTTTCTAGCTATATGTTCCTAACTTGTAACTAATGTTTACATGTGTACTACGATTACATA
AGCTTTACCCGTGTTTTATGTCGGATTGTGAGAGTGAGCTCTACTTTAACGTTCTTAATT
ACTTTTTGTTTTGTTTTGTAGAGTATTATTATTGATTTGAGTAGAGTAGTGTGAGGTTTG
GTTTGTGATGATGTTTAATAGAGTACTCTCTGGTTTTATAGGTCTGAAAAGGCAGAGAAC
AACTCAAAAAAGAGACCAAAAATGTTTTCTTGTAAATGGCTTCATGTCCGGGAAATCTAC
ATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTT
TTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTATT
   
Sequence Name 26-K105633-0022-258-F08C-OriL
GenBank Accession LN497926 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K105633-0022-258-F08C-OriL
TTCGGACTGAGCCTTTACAGAAAACATTTTTGGTCTCTTTTTTGAGTTGTTCTCTGCCTT
TTCAGACCTATAAAACCAGAGAGTACTCTATTAAACATCATCACAAACCAAACCTCACAC
TACTCTACTCAAATCAATAATAATACTCTACAAAACAAAACAAAAAGTAATTAAGAACGT
TAAAGTAGAGCTCACTCTCACAATCCGACATAAAACACGGGTAAAGCTTATGTAATCGTA
GTACACATGTAAACATTAGTTACAAGTTAGGAACATATAGCTAGAAAAGGGTATTTGGAT
TTCAATTGTCGTTTTCTTATAACGCAAAACACAAACACAAAAGGACAAAGTTGTTGACAT
ATCTCTAGTCAGGAAACAACTACTCATGTTATAACAAAAAGAAAATGTAACTTGACTGGT
TAGTAGTTTAACTCATAAAGAACCAATGCTAAATATTTCAAGTACTAAACCCATTTTTTA
AAAATGGAAAGAAAGAAAAACAGTTTACATTACACCAGGGTCGGATA

Last Updated on 06.12.2018 16:11