Sequences from confirmation amplicon


Confirmation sequences for line 399G07 and hit At1g32850 are shown.
Line ID 399G07
Locus At1g32850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:11906832
Primers used for PCR CB54 - 8474, show primer details
   
Sequence Name 34-K105706-0022-399-G07C-CB54
GenBank Accession LN501418 [GenBank]
Sequencing primer gene specific primer, CB54: 5'-AAGAAGACAATGCAAGAGGGAATA-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K105706-0022-399-G07C-CB54
AGCCTCTAGGACCAGATGACATGTGGTATTGTCCTGGTTGCAAAGAACATAGACAAGCGA
ACAAGAAGCTAGACTTGTGGAAGTTGCCAGATATTCTTGTGTTCCACCTGAAACGGTTCA
CGTATAGCAGATATTTCAAGAATAAGATTGATACGTTAGTGAACTTCCATATTCACGATC
TAGATCTTAGCAAGTACGTAAAGAACGAAGACGGTCAGTCATATCTTTACGAGCTGTATG
CGATTAGCAATCATTATGGTGGCCTTGGAGGTGGTCACTACACTGCTTACGCTAAGGTAT
TGTGTCTTTTGTTATCTATATTGATTTTGCATGCATTCCTAATAAGTCTAGTGGCAAATA
CATCGAAACTAACGTGTAGTGGCTGTTTGCTTCTGCAGTTGATGGATGAGACCAAATGGT
ATAATTTTGATGACAGCCGTGTTTCAGCTGTAAATGAATCCGAGATCAAGACTTCGGATT
GTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
   
Sequence Name 50-K105707-0022-399-G07C-8409
GenBank Accession LN507169 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K105707-0022-399-G07C-8409
CGGACTGAGCCATTTACAATTGACAATCCGAAGTCTTGATCTCGGATTCATTTACAGCTG
AAACACGGCTGTCATCAAAATTATACCATTTGGTCTCATCCATCAACTGCAGAAGCAAAC
AGCCACTACACGTTAGTTTCGATGTATTTGCCACTAGACTTATTAGGAATGCATGCAAAA
TCAATATAGATAACAAAAGACACAATACCTTAGCGTAAGCAGTGTAGTGACCACCTCCAA
GGCCACCATAATGATTGCTAATCGCATACAGCTCGTAAAGATATGACTGACCGTCTTCGT
TCTTTACGTACTTGCTAAGATCTAGATCGTGAATATGGAAGTTCACTAACGTATCAATCT
TATTCTTGAAATATCTGCTATACGTGAACCGTTTCAGGTGGAACACAAGAATATCTGGCA
ACTTCCACAAGTCTAGCTTCTTGTTCGCTTGTCTATGTTCTTTGCAACCAGGACAATACC
ACATGTCATCTGGTCCTAGAGGCTCTTCTGCTAGAAAGGCTTCCAAGCATGAAAACAAAG
ATATTCCCTCT

Last Updated on 06.12.2018 16:11