Sequences from confirmation amplicon


Confirmation sequences for line 709G06 and hit At1g33030 are shown.
Line ID 709G06
Locus At1g33030 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:11965609
Primers used for PCR CR40 - 8474, show primer details
   
Sequence Name 58-K107430-0022-709-G06C-CR40
GenBank Accession LN509723 [GenBank]
Sequencing primer gene specific primer, CR40: 5'-ACTACAATCACCTTCCCATTTGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K107430-0022-709-G06C-CR40
CTTCCGCAATGGTCATCGTCCCAGCTATGGAGCATCCACTGTTATAAGTTGTAGCAAACA
CACAAGAAACCTAGATATTATCTTTTTTTAAGTATATGATTCTGTTAAGACTTGAGAAAG
CTTTTAAAAAAGTTAACCTTCATGAAAATAGCTTCTCCTTTAGGAGTGTTTGTAAACATA
TCTCCAGCAACATGCTCAATTCCTACAACCATAAAAAGTTATGACATTAAAGATCATTTA
CTATACAACAGAATATATTCAAGAACTTGTATCTGTACCTGGAGAAGGTAATGAAGTGTT
GATAACAGTGGGCAAATCAAAGTTAATAGCTTTGATAATGTGAGTATGCTTAGAGATGAT
TCTACTGAGAAGAGAGCCATCTCCACCACCAACATCAACCAAAGACTTCACACCATCAAA
ACCATTGTAGTTCTTTAGAAATTCTTCTATGAATACTTCAAAAGAATATTATAAAATCAA
AACCAATATGAAAAAGCAAAGAATTCTTATTCATTGTTCCAATGAGCAATGGCTTCATGT
CCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTA
   
Sequence Name 42-K107429-0022-709-G06C-8409
GenBank Accession LN504483 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K107429-0022-709-G06C-8409
TGCTCATTGGAACAATGAATAAGAATTCTTTGCTTTTTCATATTGGTTTTGATTTTATAA
TATTCTTTTGAAGTATTCATAGAAGAATTTCTAAAGAACTACAATGGTTTTGATGGTGTG
AAGTCTTTGGTTGATGTTGGTGGTGGAGATGGCTCTCTTCTCAGTAGAATCATCTCTAAG
CATACTCACATTATCAAAGCTATTAACTTTGATTTGCCCACTGTTATCAACACTTCATTA
CCTTCTCCAGGTACAGATACAAGTTCTTGAATATATTCTGTTGTATAGTAAATGATCTTT
AATGTCATAACTTTTTATGGTTGTAGGAATTGAGCATGTTGCTGGAGATATGTTTACAAA
CACTCCTAAAGGAGAAGCTATTTTCATGAAGGTTAACTTTTTTAAAAGCTTTCTCAAGTC
TTAACAGAATCATATACTTAAAAAAAGATAATATCTAGGTTTCTTGTGTGTTTGCTACAA
CTTATAACAGTGGATGCTCCATAGCTGGGACGATGACCATTGCGTGAAGATACTTTCCAA
CTGTTATCAGTCGTTACCGTCAAATGGGAAGGTGATTGTAG

Last Updated on 06.12.2018 16:11