Sequences from confirmation amplicon


Confirmation sequences for line 658B07 and hit At1g33170 are shown.
Line ID 658B07
Locus At1g33170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:12029675
Primers used for PCR 21tq - 8474, show primer details
   
Sequence Name 24-K118502-0022-658-B07C-21tq
GenBank Accession LN497193 [GenBank]
Sequencing primer gene specific primer, 21tq: 5'-GTGTAACACCATTACCAGAAGCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K118502-0022-658-B07C-21tq
GAACGGCCAAACCGAGCTTTTGCGGTGCCACCTAGGATAATCGGCGGAACCATTCCGGAC
ATTAATGCTGAGAAATTCAGAGAAGACAATGAAGTGTGGAAGGAGAGAATATCATATTAC
AAACAGATAATGCCAGAGCTTTCAAGAGGAAGATTTAGGAACATAATGGACATGAATGCA
TACTTGGGAGGATTTGCTGCGGCAATGATGAAATATCCATCTTGGGTTATGAATGTGGTT
CCTGTGGATGCTGAGAAGCAAACGTTAGGGGTTATCTTTGAACGAGGATTTATAGGGACT
TATCAAGATTGGTGTGAAGGATTCTCTACGTATCCGAGAACTTATGATTTGATTCATGCT
GGTGGATTGTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGA
GTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAA
ATGTAGATGTCC
   
Sequence Name 08-K118501-0022-658-B07C-8409
GenBank Accession LN489934 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K118501-0022-658-B07C-8409
AACAATCCACCAGCATGAATCAAATCATAAGTTCTCGGATACGTAGAGAATCCTTCACAC
CAATCTTGATAAGTCCCTATAAATCCTCGTTCAAAGATAACCCCTAACGTTTGCTTCTCA
GCATCCACAGGAACCACATTCATAACCCAAGATGGATATTTCATCATTGCCGCAGCAAAT
CCTCCCAAGTATGCATTCATGTCCATTATGTTCCTAAATCTTCCTCTTGAAAGCTCTGGC
ATTATCTGTTTGTAATATGATATTCTCTCCTTCCACACTTCATTGTCTTCTCTGAATTTC
TCAGCATTAATGTCCGGAATGGTTCCCCCGATTATCCTAGGTGGCACCGCAAAAGCTCGG
TTTGGCCAATCCTCCAATGCACCGCCNGCGAATTCGTCTGAACTAGTTTGCTTCTGGT

Last Updated on 06.12.2018 16:11