Sequences from confirmation amplicon


Confirmation sequences for line 805C01 and hit At1g33540 are shown.
Line ID 805C01
Locus At1g33540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:12162152
Primers used for PCR 25fu - 8474, show primer details
   
Sequence Name 63-K111688-0022-805-C01C-25fu
GenBank Accession LN511019 [GenBank]
Sequencing primer gene specific primer, 25fu: 5'-GGTGTTTTTCGTCGTTACATTTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K111688-0022-805-C01C-25fu
TATTTTGAGGGAGGAGGCACACCGCAGAGTATAAACCAGAGGAAAGCTTCATGATGTTCC
AAAGGTGGATAAGTGGCCGACCTCTCTAGCATCTAACCGGAGAGGTGTTAATTAGTACCA
ACAAAAATCATTGAAAAGCAATGCCTAAAATACATTCATTATCACTAGGTTGGAATGTAT
CTTTCAAATTACCAGTTCAATAATCATGTATTTTATAATTCATTTACAAATAATGTAACT
CAATTCGCAAGCTTGATATCTTTGATCCCACCACCGGTTATCGCTATATTCAATTGTAAA
TGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGA
AAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCATTA
TT
   
Sequence Name 47-K111684-0022-805-C01C-8409
GenBank Accession LN506233 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K111684-0022-805-C01C-8409
ATTTCATTGAATATAGCGATAACCGGTGGTGGGATCAAGATATCAAGCTTGCGAATTGAG
TTACATTATTTGTAAATGAATTATAAAATACATGATTATTGAACTGGTAATTTGAAAGAT
ACATTCCAACCTAGTGATAATGAATGTATTTTAGGCATTGCTTTTCAATGATTTTTGTTG
GTACTAATTAACACCTCTCCGGTTAGATGCTAGAGAGGTCGGCCACTTATCCACCTTTGG
AACATCATGAAGCTTTCCTCTGGTTTATACTCTGCGGTGTGCCCTCCTCCCTACAAAATT
AAATCAAGGAAAAACATTTTTCAAAAAATGTAACGACGAANAACAC

Last Updated on 06.12.2018 16:11