Sequences from confirmation amplicon


Confirmation sequences for line 583B01 and hit At1g33810 are shown.
Line ID 583B01
Locus At1g33810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:12265134
Primers used for PCR 08pf - 8474, show primer details
   
Sequence Name 20-K115157-0022-583-B01C-08pf
GenBank Accession LN495338 [GenBank]
Sequencing primer gene specific primer, 08pf: 5'-ACACAGAACAGTACCAAAGTGGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K115157-0022-583-B01C-08pf
ATCTTTTTCCTTTCTCCAATACCCAATAATCTAAAGATGTGTGAACAAATGACAGAAGAA
AGATTGCCACAATTTACTAGTAATTTCACAATTTCCTAAGAACAGAGCAGAGAGGAGCAA
ATTCACTATATGAAACCTAGAATTACAGTAATGAGAGAAGGGTAGACCTGATAGTTGCGA
TTGAAGCTGTAACGAAGCTGAGGATCAATCTCAAGTGAATCATCATCATCATCAGCGAGA
CTTCCTTTTTTGGTTCCGCGGCTAGGGACGTGCATCTTCGACATACCATCAGAATCCTTT
GTGACTTTAAGCTTTGCGTTGCTAAACTTATCGGAATCATCTTCGACATACCATCAGGAT
ATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGA
TGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
ATGTCCGCA
   
Sequence Name 08-K115156-0022-583-B01C-8409
GenBank Accession LN489852 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K115156-0022-583-B01C-8409
TTTCATTGAATATATCCTGATGGTATGTCGAAGATGATTCCGATAAGTTTAGCAACGCAA
AGCTTAAAGTCACAAAGGATTCTGATGGTATGTCGAAGATGCACGTCCCTAGCCGCGGAA
CCAAAAAAGGAAGTCTCGCTGATGATGATGATGATTCACTTGAGATTGATCCTCAGCTTC
GTTACAGCTTCAATCGCAACTATCAGGTCTACCCTTCTCTCATTACTGTAATTCTAGGTT
TCATATAGTGAATTTGCTCCTCTCTGCTCTGTTCTTAGGAAATTGTGAAATTACTAGTAA
ATTGTGGCAATCTTTCTTCTGTCATTTGTTCACACATCTTTAGATTATTGGGTATTGGAG
AAATGTGAAAAAGATTTTATCTTTTATGTTTCTGCACCACTTTGGTCTGTTTCTGTG

Last Updated on 06.12.2018 16:11