Sequences from confirmation amplicon


Confirmation sequences for line 777G05 and hit At1g35580 are shown.
Line ID 777G05
Locus At1g35580 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:13122610
Primers used for PCR CB16 - 8474, show primer details
   
Sequence Name 63-K104528-0022-777-G05C-CB16
GenBank Accession LN510986 [GenBank]
Sequencing primer gene specific primer, CB16: 5'-TTGAATTTGAGTGGAGGCTAACTT-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K104528-0022-777-G05C-CB16
CCCTATTCGCCNATAAGAATCGGTCATTTCAGCTTATTGAAAGATATTCATATTGCAATA
GCGCCAAAATGCAAATTATTAAAAAAAAAGGTGTAATTTCGATAAAAACGGCAAACGAGC
TTTTCCCATTTTCTTTTTCCTTACTTGCAAAGCAAGAAAGAAAGCAATCAATAAAAAGTC
TATGTCCCCGCACAAGTTTTCAATAACATTATCATACAAACAACAACAACAAAAAATCAT
ATCGTGGCAAATATTAGAACCGGAACAAAAACCTAAAGCGGTTTTTCAGAGTTGTGGCCA
AGACGCAGATCGCTTGATGACTGGTTTCATGAGTTTGTCTTCTTCAAGAGAGATCATACC
AATGTGTGAAGGATCTTCTAGCAACATTTTCGCCACTAAGTAACCTGCGATTGACCAAGT
CTGGTATTTCCTTGCCTGCAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGA
GTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAA
ATGTAGATGTCCGCAGCGTTATT
   
Sequence Name 79-K104529-0022-777-G05C-8409
GenBank Accession LN513824 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>79-K104529-0022-777-G05C-8409
GCCATTGCGGCAAGGAAATACCAGACTTGGTCAATCGCAGGTTACTTAGTGGCGAAAATG
TTGCTAGAAGATCCTTCACACATTGGTATGATCTCTCTTGAAGAAGACAAACTCATGAAA
CCAGTCATCAAGCGATCTGCGTCTTGGCCACAACTCTGAAAAACCGCTTTAGGTTTTTGT
TCCGGTTCTAATATTTGCCACGATATGATTTTTTGTTGTTGTTGTTTGTATGATAATGTT
ATTGAAAACTTGTGCGGGGACATAGACTTTTTATTGATTGCTTTCTTTCTTGCTTTGCAA
GTAAGGAAAAAGAAAATGGGAAAAGCTCGTTTGCCGTTTTTATCGAAATTACACCTTTTT
TTTTAATAATTTGCATTTTGGCGCTATTGCAATATGAATATCTTTCAATAAGCTGAAATG
ACCGATTCTTATTGGTGTAATAGGTGAAATTATCTTTATTATAATCATGATTTGAAGTTA
GCCTCCACTCAAATTCAAATCNTGATTATAATAAAGATAATTTCACCTATTACACCAATA
AGAATCGGTCATTTCGGCTTATTGAAAGATATTCATATTGCAATAGCGCCAAAATGCAAA
TTATTAAAAAAAGAGGTG

Last Updated on 06.12.2018 16:11