Sequences from confirmation amplicon


Confirmation sequences for line 541B10 and hit At1g42990 are shown.
Line ID 541B10
Locus At1g42990 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:16135855
Primers used for PCR KQ40 - 8474, show primer details
   
Sequence Name 76-K101672-0022-541-B10C-8409
GenBank Accession LN513366 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K101672-0022-541-B10C-8409
GCTTTATCAATCCATTTTGAAGTTATGCCTCAAAATCAACATTTTAAAGGCAGAAGCATT
ATTAGATAGATATCATCCATAACCATAAGACCCACATTCACTGTTAACTTATCGATATTA
GACAGAAAACTTTCAACAGCGAAATACAAAACAAGAGAGAGAAAACTAAAAGACACTCAT
CTACAACTCAAAATGCGCAAAAGAAGCAGTTAAAAAAGGCGTTGTCACGCCGCAAGGGTT
AAGATTTGGTATTTCATCCTAGGCCTCGAACCCTTACATCTCCGACTAACGCCGGTATAA
GACGGTTTGCTACTACTCCCGAGCCCGTTTAGAACCAGCTTTTCTGGTTCTGGACGTAGG
AGGCAACACTTTGTGTGGGACATATNAGGGAATAGGCAAATGAAGTTTACTCCCAGAA
   
Sequence Name 76-K101679-0022-541-B10C-KQ40
GenBank Accession LN513367 [GenBank]
Sequencing primer gene specific primer, KQ40: 5'-GCTTCTGGGAGTAAACTTCATTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K101679-0022-541-B10C-KQ40
AAGTGTTGCCTCCTACGTCCGAACCGAAAAGCTGGTTCTAACGGGCTCGGGAGTAGTAGC
AAACCGTCTTATACCGGCGTTAGTCGGAGATGTAAGGGTTCGAGGCCTAGGATGAAATAC
CAAATCTTAACCCTTGCGGCGTGACAACGCCTTTTTTAACTGCTTCTTTTGCGCATTTTG
AGTTGTAGATGAGTGTCTTTTAGTTTTCTCTCTCTTGTTTTGTATTTCGCTGTTGAAAGT
TTTCTGTCTAATATCGATAAGTTAACAGTGAATGTGGGTCTTATGGTTATGGATGATATC
TATCTAATAATGCTTCTGCCTTTAAAATGTTGATTTTGAGGCATAACTTCAAAATGGATT
GATAAAGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATG
GAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATTTCCG

Last Updated on 06.12.2018 16:11