Sequences from confirmation amplicon


Confirmation sequences for line 025G04 and hit At1g44020 are shown.
Line ID 025G04
Locus At1g44020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:16717662
Primers used for PCR DC69 - 8474, show primer details
   
Sequence Name 54-K032605-022-025-G04C-8409
GenBank Accession LN508381 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K032605-022-025-G04C-8409
ATTTTCGGCTCCAATCGGTATAATCAATGATAAAAATTATTTGAGCAATGAGTGTATACA
GCTCTGAATCTCGATCCAAATAGTAGAGAAGAGAAAACGTGTGTGTAAAGAGTGCCACTA
GCTGCATTGGTGGCTCCCACTCTGGTTCTAGATCTATAGAGCTAATGAGACGGATCATTT
CAGTCATGCTTGATATGAACTGCCACTCTGGTTCTGAATTTGATATAGAGTTGGCAAAAG
ACATTATTTGCTTAACGACTGCTACCACTGAAATGAACTCCGACTCTGGCTCCGGCTCTG
AATAATCCAAATCAAGAGAGCGTACGAGAGTGATTACTTTACTTGTAAGCGATATGAATG
ATTAATTTTT
   
Sequence Name 54-K032605-022-025-G04C-DC69
GenBank Accession LN508382 [GenBank]
Sequencing primer gene specific primer, DC69: 5'-TTGCCAAAATTCAAATCATTCATA-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K032605-022-025-G04C-DC69
TCTCGTAGCTCTCTTGATTTGGATTATTCAGAGCCGGAGCCAGAGTCGGAGTTCATTTCA
GTGGTAGCAGTCGTTAAGCAAATAATGTCTTTTGCCAACTCTATATCAAATTCAGAACCA
GAGTGGCAGTTCATATCAAGCATGACTGAAATGATCCGTCTCATTAGCTCTATAGATCTA
GAACCAGAGTGGGAGCCACCAATGCAGCTAGTGGCACTCTTTACACACACGTTTTCTCTT
CTCTACTATTTGGATCGAGATTCAGAGCTGTATACACTCATTGCTCAAATAATTTTTATC
ATTGATTATACCGATTCGGAGCCGAAAAATCTACATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGAC
AAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAA
TCTTTATTTCGACGTGTCTACATTC
Primers used for PCR FP26 - 8474, show primer details
   
Sequence Name 82-K035342-022-025-G04C-FP26
GenBank Accession LN514142 [GenBank]
Sequencing primer gene specific primer, FP26: 5'-CTTGTCTTTGAAACAGAGCAAGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>82-K035342-022-025-G04C-FP26
GACTGGTGGCTTCTCTGCACAAGCTATGTTCATATCATAATCGCAACCCGTGCAATAATA
GAATATCTTTTTGAGATCTTCATCACAACAAAAGCATTTTCTGGTTTGAATTATCGACGA
CTTCTGTGAGACAAGCTGAAGAGAATGCTTTGGATGTAGAAGGTGTTTTACCTCAACTGG
TGTCTTTTCACATTCTATATGGTCCTCGCCGTTGCAATCTTTGCAAATAAAGTGGGAAGC
CTCCTCTCCTTTCGCTAAGTAGAGTCTATGCCGGTTTTCGGGTTGACATTTCCCCTTTTC
TTTGTTCTTCCGCTCGATTTTTCCCGTTACATGAAATTTTCCTTGTACAAGTTGTACTTT
CCATTGAGGGGACAAGGACATAAGCTGATCTATGGATCAATTGTAAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAA
GTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAAC
AAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACGT
   
Sequence Name 82-K035342-022-025-G04C-8409
GenBank Accession LN514141 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>82-K035342-022-025-G04C-8409
ATTCCGGACATAAGCCATTTACAATTGATCCATAGATCAGCTTATGTCCTTGTCCCCTCA
ATGGAAAGTACAACTTGTACAAGGAAAATTTCATGTAACGGGAAAAATCGAGCGGAAGAA
CAAAGAAAAGGGGAAATGTCAACCCGAAAACCGGCATAGACTCTACTTAGCGAAAGGAGA
GGAGGCTTCCCACTTTATTTGCAAAGATTGCAACGGCGAGGACCATATAGAATGTGAAAA
GACACCAGTTGAGGTAAAACACCTTCTACATCCAAAGCATTCTCTTCAGCTTGTCTCACA
GAAGTCGTCGATAATTCAAACCAGAAAATGCTTTTGTTGTGATGAAGATCTCAAAAAGAT
ATTCTATTATTGCACGGGTTGCGATTATGATATGAACATAGCTTGTGCAGAGAAGCCACC
AGTCTTATTTATAGACCGTCCCAAGTGGCATGCAGATACACTTGCTCTGTTTCAAAGACA
Primers used for PCR FP26 - 8474, show primer details
   
Sequence Name 49-K118471-0022-025-G04C-FP26
GenBank Accession LN506989 [GenBank]
Sequencing primer gene specific primer, FP26: 5'-CTTGTCTTTGAAACAGAGCAAGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K118471-0022-025-G04C-FP26
GCTTTCTCTGCACAGCTATGTTCATATCATAATCGCAACCCGTGCAATAATAGAATATCT
TTTTGAGATCTTCATCACAACAAAAGCATTTTCTGGTTTGAATTATCGACGACTTCTGTG
AGACAAGCTGAAGAGAATGCTTTGGATGTAGAAGGTGTTTTACCTCAACTGGTGTCTTTT
CACATTCTATATGGTCCTCGCCGTTGCAATCTTTGCAAATAAAGTGGGAAGCCTCCTCTC
CTTTCGCTAAGTAGAGTCTATGCCGGTTTTCGGGTTGACATTTCCCCTTTTCTTTGTTCT
TCCGCTCGATTTTTCCCGTTACATGAAATTTTCCTTGTACAAGTTGTACTTTCCATTGAG
GGGACAAGGACATAAGCTGATCTATGGATCAATTGTAAATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGAT
   
Sequence Name 17-K118470-0022-025-G04C-8409
GenBank Accession LN494065 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K118470-0022-025-G04C-8409
ATTTACATTGATCCATAGATCAGCTTATGTCCTTGTCCCCTCAATGGAAAGTACAACTTG
TACAAGGAAAATTTCATGTAACGGGAAAAATCGAGCGGAAGAACAAAGAAAAGGGGAAAT
GTCAACCCGAAAACCGGCATAGACTCTACTTAGCGAAAGGAGAGGAGGCTTCCCACTTTA
TTTGCAAAGATTGCAACGGCGAGGACCATATAGAATGTGAAAAGACACCAGTTGAGGTAA
AACACCTTCTACATCCAAAGCATTCTCTTCAGCTTGTCTCACAGAAGTCGTCGATAATTC
AAACCAGAAAATGCTTTTGTTGTGATGAAGATCTCAAAAAGATATTCTATTATTGCACGG
GTTGCGATTATGATATGAACATAGCTTGTGCAGAGAAGCCACCAGTCTTATTTATAGACC
GTCCCAAGTGGCATGCAGATACACTTGCTCTGTT

Last Updated on 06.12.2018 16:11