Sequences from confirmation amplicon


Confirmation sequences for line 214C10 and hit At1g44940 are shown.
Line ID 214C10
Locus At1g44940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:16994664
Primers used for PCR 10oc - 8474, show primer details
   
Sequence Name 29-K114720-0022-214-C10C-10oc
GenBank Accession LN499405 [GenBank]
Sequencing primer gene specific primer, 10oc: 5'-AATCATGTTCACTTCCTTCCATTC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K114720-0022-214-C10C-10oc
CAACGACAGTAGTAAACTTTCTCCTGATTAGGTGATGAATCTTCAACCTTACCAGCAAAT
GTCTTTTTTTATCCTTCCTCTTTATACTAATTTGATTTTCCCAGCAATGACCTTAGAAAG
AAGGTGCTCTCGATTATTGACATGTGTGGTCTTAGGTTGTTGGCTGTTGCTTTTCTAGGT
ACTTGTTTGACATTGATACTCATAGTTTCAGTCTTCTTTTGTTATTCTTTTCTGCGTGGA
AACAAATGTTCAATTGTATGTGTTCTTATTTCCCTTTTTAGGTGGTACTAGATAATTCGA
AGGAAAGCCCTGGTGCATGGTGCAACAACATATGGTAATTTGTTAGCAATACTTAAATAT
ACTTTTTGACACTAAAGCAACAGAAAAATGGCTTCATGTCCGGGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGTAGAT
   
Sequence Name 09-K114719-0022-214-C10C-8409
GenBank Accession LN490294 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K114719-0022-214-C10C-8409
AAAGTATATTTAAGTATTGCTAACAAATTACCATATGTTGTTGCACCATGCACCAGGGCT
TTCCTTCGAATTATCTAGTACCACCTAAAAAGGGAAATAAGAACACATACAATTGAACAT
TTGTTTCCACGCAGAAAAGAATAACAAAAGAAGACTGAAACTATGAGTATCAATGTCAAA
CAAGTACCTAGAAAGCAACAGCCAACAACCTAAGACCACACATGTCAATAATCGAGAGCA
CCTTCTTTCTAAGGTCATTGCTGGGAAAATCAAATTAGTATAAAGAGGAAGGATAAAAAA
AGACATTTGCTGGTAAGGTTGAAGATTCATCACCTAATCAGGAGAAAGTTTACTACTGTC
GTTGATGTAAGTCAAAGCAGTTCTCTTATCCACATGGTTGAATGGAAG

Last Updated on 06.12.2018 16:11