Sequences from confirmation amplicon


Confirmation sequences for line 834B05 and hit At1g48840 are shown.
Line ID 834B05
Locus At1g48840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:18063062
Primers used for PCR 60us - 8474, show primer details
   
Sequence Name 62-K118051-0022-834-B05C-60us
GenBank Accession LN510850 [GenBank]
Sequencing primer gene specific primer, 60us: 5'-CTGCTAGCCAGACATTACTCAGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K118051-0022-834-B05C-60us
CCTTATATTATTAAACATGGAAGCAGGTTGATGAAGATCCAACCGTTGGTTTAGAAGCCT
TCTCACGGATTGCTCCAGCAGTTCCGATAATAGCTGATGTGATCATATGTGAGAATTTGT
TTGGGATACTGACTTCCGTCTCAAACAGTGTTCGGCTTCAGTTTTATGTTTATGACAAGT
ATTTGTATGGACTTGAAAGGTAAATATAATCTTTCATCCTTATAAACATTCTCCAGAAGA
ACATTGTGAATCTGAATCCGGGATCACTTTCTAGAGCAATAAAGAAGATGAAGAGCCAGT
CCGAGTCATCTTTACTTTCTGGTGTTCGATCAAAAGGAGAAAAGATTCTTGAGCTCGATG
GTACAGTTACTACCCAACCAGTTCTTGAACATATTGGAATATCTACATGGCCAGGTATGT
TTTTCTCTGCACAAGCAAAGTGTGAAAATTCTCCTCTCTATTTGGTTGATTGAATTTAGT
ATTGTTAAATGCACTATATTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAA
TCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACC
AATTTTTTTTCAATTCAAAAATGTAGATGTC
   
Sequence Name 26-K118050-0022-834-B05C-8409
GenBank Accession LN498121 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K118050-0022-834-B05C-8409
TGAATATAGTGCATTTAACAATACTAAATTCAATCAACCAAATAGAGAGGAGAATTTTCA
CACTTTGCTTGTGCAGAGAAAAACATACCTGGCCATGTAGATATTCCAATATGTTCAAGA
ACTGGTTGGGTAGTAACTGTACCATCGAGCTCAAGAATCTTTTCTCCTTTTGATCGAACA
CCAGAAAGTAAAGATGACTCGGACTGGCTCTTCATCTTCTTTATTGCTCTAGAAAGTGAT
CCCGGATTCAGATTCACAATGTTCTTCTGGAGAATGTTTATAAGGATGAAAGATTATATT
TACCTTTCAAGTCCATACAAATACTTGTCATAAACATAAAACTGAAGCCGAACACTGTTT
GAGACGGAAGTCAGTATCCCAAACAAATTCTCACATATGATCACATCAGCTATTATCGGA
ACTGCTGGAGCAATCCGTGAGAAGGCTTCTAAACCAACGGTTGGATCTTCATCAACCTGC
TTCCATGTTTGAAAATATAAGGACCAAGTCTTGACTCAATAGACCACAGACACAGAAATC
AAAAGTCCTTACTTACACTGAGT
Primers used for PCR 67wa - 3144, show primer details
   
Sequence Name 75-K126264-0022-834-B05C-67wa
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 67wa: 5'-GTGCAATGTCCCTGATAAAAGAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K126264-0022-834-B05C-67wa
TACGTAATTAATGTTACCTTCTTTGGCTTTGGCAGTTCGGTCAAGTACTCTAGAACTCGA
CATCTCTGCAAGCGTCTCCAGTATACGATCTCCACCAGGAAGCTGATCGCAGAGATTGAA
CGGAAGAAGATTCTCGTAACGCACAGGGTTTGTCAAACCAACTTCTTGAATGGCCTGCAC
GCGTAATATGCCGAGGACAGCTTTTGAGATAGCTTCATCTTTCGCCACACTGTTGATCTT
GAACTTCTTTATGTATCTGTGAACATATAACACTTCTAGGATTATCGCGAGCCAGTAATC
TCTTCGTGTGTGACCCTTAAGCTCAGGGAACTCCATTACTACTGGTTCTGGTCTACAGAA
CAAGAGACATTAAAATATGTAACTGAACCAGTAAGAAAAGAAGTGAATACAAAACAAGTC
ATAATAACATCATTCCTTACAGGGAAATAGACTTGTAAGAGACGGCCTTATCAAAAAGCC
GAGTCCCCCAAGGACCGGTTAACTCGGGTTTAATGACTTGTTTCAGATCATCAGATAGGC
TATAACGCTTTGGTGTGTCGAAGGATACAACCTTTATAGCCTCAAAATATAGTGAATGAT
CAGTAAGAATCAAGCGACCTGGTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGG
TACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGA
TAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCAC
   
Sequence Name 65-K126263-0022-834-B05C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K126263-0022-834-B05C-8409
ACCGGACCTTGGGGGACTCGGCTCATTGAGAAGGCCTTCTCCTCCAAGACTATTTCGCTG
ACAGGAATCATGTTATTGAGACTTGCTTAGCATTCACCTCTTTTCTTACT

Last Updated on 06.12.2018 16:11