Sequences from confirmation amplicon


Confirmation sequences for line 854B04 and hit At1g51880 are shown.
Line ID 854B04
Locus At1g51880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:19273272
Primers used for PCR EU82 - 8474, show primer details
   
Sequence Name 02-K103845-0022-854-B04C-EU82
GenBank Accession LN487003 [GenBank]
Sequencing primer gene specific primer, EU82: 5'-TCATAGGGGTTACTTGTATCGACC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K103845-0022-854-B04C-EU82
ATTCATCCTTACCAAACTGTCGAACCCAGACTCGATCATGGATATCCTCATCATACCTAT
AGAAATGAGAGGAAAAGCCACTAGACTTGTTTTTGTTAACTCAAAAACAAATAACAAAAA
AAAGAAAGTCCCGTCGTTAAATGCAACATCCAGATCTAGCCGTTAATGTTGTTCGTTTCT
AAAATCGCTTTTTCCATATGCATGTTGCATTTGGATAAAACGTTTTGCATCCGAAAATCC
GAATGCAAATACCAAATTTTTCAAACTAGCTAGCAATATCTGGTGCACTTTACATCAGGA
TATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATG
ATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTA
GATGTCCGCAGCGTTATT
   
Sequence Name 18-K103846-0022-854-B04C-8409
GenBank Accession LN494275 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K103846-0022-854-B04C-8409
GCCATTTACATTGAATATATCCTGATGTAAAGTGCACCAGATATTGCTAGCTAGTTTGAA
AAATTTGGTATTTGCATTCGGATTTTCGGATGCAAAACGTTTTATCCAAATGCAACATGC
ATATGGAAAAAGCGATTTTAGAAACGAACAACATTAACGGCTAGATCTGGATGTTGCATT
TAACGACGGGACTTTCTTTTTTTTGTTATTTGTTTTTGAGTTAACAAAAACAAGTCTAGT
GGCTTTTCCTCTCATTTCTATAGGTATGATGAGGATATCCATGATCGAGTCTGGGTTCGA
CAGTTTGGTAATGGATTGAAATCGATAAGCACAGACCTTTTGGTCGATACAAGTAACCCC
TATA
Primers used for PCR BB86 - 3144, show primer details
   
Sequence Name 51-K104831-0022-854-B04C-3144
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K104831-0022-854-B04C-3144
ATCCACTATCTTCGCAGACCCTTCCTCANATAAGGAAGTTCATTTCATTTGGACAGGACA
GGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTATCGTTGCATCTACACGCA
GAAACGAACCAGAAAAAATACAAAAGAAAAAAAAAAAAAAAAAAAACCCCTTTTCCTTTA
AAAAGGTGGGAGGGGCAAAAAAAAAAACTTTGGGAAACCCAATAAAGAACCCTTTTGGGG
GGGGGAAATTTTTTTGGTCCAAGGGAAAAATTCCCCCCAAAAAAAAAAGGGGTTCCCTTT
TCCGGGTTTAAAAAAAAAAATTTTAAAAAGGGTTTGGGGTAAAAAAAGAAATCTTCCGGG
GGAGGGCCCCTTTTCTAACCCCTCCAAATTAAAAAAAATCCCTTTTGCGGGGCCCATGGG
AAATTTAAAATTGGGGGATTTGTCCAAACCCTCCAAATTTCCCAACCTAAAGGGTGGCCC
TAAAAAAAATTCGGTTTTTAGGGGAAGGGTAAAATTG
   
Sequence Name 81-K118835-0022-854-B04C-BB86
GenBank Accession LN514122 [GenBank]
Sequencing primer gene specific primer, BB86: 5'-CAACTTCAACCTTACCGCTAAAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>81-K118835-0022-854-B04C-BB86
CACTTTACGTATGGGATACGATGGTTTGAGACAAGTCCCCAAGTTTGATATTCACATTGG
TCCTAGCAAATGGACTTCTGTTAAGTTGGATGGAGTAGGAAATGGTGCAGTCCTCGAGAT
GATTCATGTCTTAACACAAGACCGTCTTCAAATTTGTCTTGTTAAGACAGGAAAAGGGAT
ACCATTTATTTCGTCGCTGGAACTTCGTCCGTTGAACAATAATACTTACCTCACGCAAAG
TGGATCGTTGATTGGGTTCGCAAGAGTTTTCTTTTCAGCCACTCCAACATTCATAAGGTA
AAGAGGTTTTTTTTTTTTTTTTTTTTTTTTTGGATTTTTTCGGGGTCCTTTCCGGCGGGA
AAAGCCAAAAAAAACCCAAGGGGGGAAAAAAAAATTTTAACCCCGGGGACCCTGGCCCTC
CCAAAAAAAAAAAACTTCCTTTTTTAAAAAAAGGGTTTTTCAAAAAAAAAGGGGATTTGG
GCTCCCCCCTTTCCTCGGGGGAAAATTTCAATTCACCCCAA

Last Updated on 06.12.2018 16:11