Sequences from confirmation amplicon


Confirmation sequences for line 347E12 and hit At1g52630 are shown.
Line ID 347E12
Locus At1g52630 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:19606695
Primers used for PCR 07ya - 8474, show primer details
   
Sequence Name 27-K119925-0022-347-E12C-07ya
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 07ya: 5'-AATCAGATGAGACGCGATGTAAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K119925-0022-347-E12C-07ya
GTTATTCATCAGGACTTCAACTTGTATTATATCTGCCTTAGGAAGTAAATCATATGATGA
TGTGATGCTGCTTGCAGTTGTGTGATGGAGTTGGGATTGCCCGTTTGTTGAATGCTACAC
TTGTTCTGCCAAAGTTTGAGGTAGCTGCCTATTGGAATGAGTCAAGGTACTCCTACTTTC
TCTTGAAACTTAGATAACACAATTCCAAGCGTGTGCTTCGTTAAATTAATCAAGGAAGAG
ACTAATCAGTGATATTTGTTGATGCAGTGGATTTGCAGATGTGTTTGATGTAGACTACTT
TATTCAAAAGATGAGTGGTTATATCGAAGTTGTTAAAGAATTGCCAAAAGATATTGCATC
AAAAGAACCATTCAAAGTAGACTGCAGCAAAAGGAAGGGTCAATTTGATTACATTGAAAG
TGTTCTTCCACTGTTGTTGGAACATCATTACATTTCTTTTACACCAGCAATGAGCCAACG
CAGGGACAGGTGCCATTCTAATAGACTATAAAAGCCTTTGTCTGTAGTTGTTATTTCTAA
AATATTTCAAGTATCTTACTCTTCTACGTTGATAAAATACATTTTCAGGTATCCCGAATA
TGCAAGAGCCACTCTGTGTCAAGCCTGTTATTCGGCTATACACCTTACTAGTTCCTTGGA
GAAGAAGGCCGTCGAGCTTTTTGATGCTATTCCCAAACCCTTCTTGTCGCTTCACCTCAG
ATTTGAACCTGACATGGTAGCGTACAGCCNATGTGAATACCCAAACCTCTCTCCTTCATC
TATCGCTGCCATTGAAGCTGCTCGTGCTGATAGGAAGCCATGGACTGGAGAACTAGCTCA
GACATGGAGAAAGCGAGGCAAATGCCCTCTGACTC
   
Sequence Name 11-K119924-0022-347-E12C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K119924-0022-347-E12C-8409
TTGAATATATCCTGATATGCGTCACTATTGATGGACACGTGATAATCCAATGCAGCTTTT
GTGTTTCCATGCATTCTGGTGAAATCCTCTTGGTTAAGTAGAACAGACTTTGTGAACACA
TTTGTATAAACGGATGTGAATCCTTCCATCTCCATTAGACCGTCTCCAGCTGCTAAGTAT
ATGTTTGTGCTTGTTGGGATGTTAAGTGACTGAAGCATCAACACTGTTTCATTAGGAGTC
AGAGGGCATTTGCCTCGCTTTCTCCATGTCTGAGCTAGTTCTCCAGTCCATGGCTTCCTA
TCAGCACGAGCAGCTTCAATGGCAGCGATAGATGAAGGAGAGAGGTTTGGGTATTCACAT
TGGCTGTACGCTACCATGTCAGGTTCAAATCTGAGGTGAAGCGACAAGAAGGGTTTGGGA
ATAGCATCAAAAAGCTCGACGGCCTTCTTCTCCAAGGAACTAGTAAGGTGTATAGCCGAA
TAACAGGCTTGACACAGAGTGGCTCTTGCATATTCGGGATACCTGAAAATGTATTTTATC
AACGTAGAAGAGTAAGATACTTGAAATATTTTAGAAATAACAACTACAGACAAAGGCTTT
TATAGTCTATTAGAATGGCACCTGTCCCTGCGTTGGCTCATTGCTGGTGTAAAAGAAATG
TAATGATGTTCCAACAACAGTGGAAGAACACTTTCAATGTAATCAAATTGACCCTTCCTT
TTGCTGCAGTCTACTTTGAATGGTTCTTTTGATGCAATATCTNTTGGCAATTCTTTAACA
ACTTCGATATAACCACTCATCTNTTGAATAAAGTAGTCTACATCAAACACATCTGCAAAT
CCACTGCATCAAAAATATCACTGAATAGTCTCTTCCTTGATTAATTTACGAAGCACACGC
TTGGAATTGTGTTATCNTAGTTTCAAGAGAAAGTAGGAGTACCTTGACTCAATCCATAGG
CAGCTACCT

Last Updated on 06.12.2018 16:11