Sequences from confirmation amplicon


Confirmation sequences for line 426C01 and hit At1g52827 are shown.
Line ID 426C01
Locus At1g52827 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:19671857
Primers used for PCR KT39 - 8474, show primer details
   
Sequence Name 69-K034239-022-426-C01C-8409
GenBank Accession LN512139 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K034239-022-426-C01C-8409
AAGCCATTTACAATTGAATACTCATAGTAAGTCATTTCTTGTTGTGGCGGTGCCTTCATT
CTTTTGCGTGAGTGTCTTGATAACTTTAGGGGTTGGGATTAATTGGATGTAGTATATATA
TAGACTTCAAATCTGCTACCTGCAAACTGATATGATCATTTAATACGGAAGATATGGCAA
CATTTATTTGGACCGACAAAGTGGTTAGAATCTTCACCCAAAACTACATTTGATATGATA
TAGGAATAAGCCAGCTTTAATTTGGCTCTTGGTGAAAGAAAAAAACAAATATTTAACATT
TTTCATTCTTTCTTAAATTAGTTGGGAAGATTCATTAGGTTTTCACTATTTTTAGCTCCG
TTTAAGATTCATAGGTTTCCACTATCTTTGGATCGGCGATAAAAAGATGATTTAGCCGGA
NGGATTTA
   
Sequence Name 70-K034239-022-426-C01C-KT39
GenBank Accession LN512325 [GenBank]
Sequencing primer gene specific primer, KT39: 5'-ATAAATCCTCCGGCTAAATCATCT-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K034239-022-426-C01C-KT39
GTGGGAACCTATGAATCTTAAACGGAGCTAAAAATAGTGAAAACCTAATGAATCTTCCCA
ACTAATTTAAGAAAGAATGAAAAATGTTAAATATTTGTTTTTTTCTTTCACCAAGAGCCA
AATTAAAGCTGGCTTATTCCTATATCATATCAAATGTAGTTTTGGGTGAAGATTCTAACC
ACTTTGTCGGTCCAAATAAATGTTGCCATATCTTCCGTATTAAATGATCATATCAGTTTG
CAGGTAGCAGATTTGAAGTCTATATATATACTACATCCAATTAATCCCAACCCCTAAAGT
TATCAAGACACTCACGCAAAAGAATGAAGGCACCGCCACAACAAGAAATGACTTACTATG
AGTATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATG
ATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTA
GATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATC
CGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCG
ACGTGTCTACATTCACTCCCAAATGG

Last Updated on 06.12.2018 16:11