Sequences from confirmation amplicon


Confirmation sequences for line 166D07 and hit At1g54390 are shown.
Line ID 166D07
Locus At1g54390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:20305526
Primers used for PCR BD45 - 8474, show primer details
   
Sequence Name 37-K028098-022-166-D07C-8409
GenBank Accession LN502491 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K028098-022-166-D07C-8409
TAGAGATAGAGACCGGGATCGTGACAGGGAATTTGAGCTCATGCCTCCTCCAGGAAGCAA
TCGGAAAGACCTTATGCCAATTGAAGAGCAGCCAATCGATCCAAACGAACCAACTTACTG
TGTCTGCCATCAGGTCCTGAATACATAAATTTTGCANAATCTGTAAGTTGCCTGATCCTG
TTGTGATCTTTGGTTACATTTT
   
Sequence Name 37-K028098-022-166-D07C-BD45
GenBank Accession LN502492 [GenBank]
Sequencing primer gene specific primer, BD45: 5'-TAGCTACAGAATGCATGGAAATTG-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K028098-022-166-D07C-BD45
GTAAGCATACCTTCTCACAGGTACAAATGATATTAGTGTGACTGTGGGAGGAGTCTGCAG
GTGGGGCAGTACCATTTCCCTTTGAATCTGGTCTCAGGTGTGAGGCCAACGCATGTATAG
TGAAACCATTCACCTCCTTGGCACTGTCGAAACAGGAACAAAAGGTTTCATCATAACAGC
TATACATTAAAAAATAAATAATAAGATGCTGGGAGAGCAGGCTCACATTCTCATTGTCAC
AGGCAATCATGTCTCCAAAGGACACCTGAAACAAATAACGAAAATGTTAACCAAAGATCA
CAACAGGATCAGGCAACTTACAGATTTTGCAAAATTTATGTATTCAGGACCTGATGGCAG
ACACAGTAAGTTGGTTCGTTTGGATCGATTGGCTGCTCTTCAATTGGCATAAGGTCTTTC
CGATTGCTTCCTGGAGGAGGCATGAGCTCAAAATCCCTGTCACGATCCCAGTCTCTATCT
CTATAATCAATCTTCTTGTTGCCATTCATATTCAATTGTAAATGGCTTCATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACA
TTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATT
ATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACA

Last Updated on 06.12.2018 16:11