Sequences from confirmation amplicon


Confirmation sequences for line 694B11 and hit At1g56030 are shown.
Line ID 694B11
Locus At1g56030 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:20956911
Primers used for PCR 29jn - 8474, show primer details
   
Sequence Name 58-K113934-0022-694-B11C-29jn
GenBank Accession LN509766 [GenBank]
Sequencing primer gene specific primer, 29jn: 5'-GAGTTTAAATGGACCAACAAAAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K113934-0022-694-B11C-29jn
CTATTCCTTACTCGTTGAAAATATAGACTATCTCTCTGAATAAAGAGATCTCAAAGAGTT
GTGTTACGTCACATTGTCCGATTGTCACGTGATTCTTTTTTTTTTCATAGCTAGCGAAAA
AAGTGGGAACATATTAATTTCTTTAATATTTCTGGGTCTTTCGTATGTTTGGCTTTTCAT
GGCGTTTTAGGTGTGCCTACAAAAGCACACTTGTCTTCTTTATCGTTCTGAGAAAAAATG
GCTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCACCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TTTCCG
   
Sequence Name 23-K113933-0022-694-B11C-oriL
GenBank Accession LN496641 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K113933-0022-694-B11C-oriL
ATTTACATTGAAGCCATTTTTTCTCAGAACGATAAAGAAGACAAGTGTGCTTTTGTAGGC
ACACCTAAAACGCCATGAAAAGCCAAACATACGAAAGACCAAGAAATATTAAAGAAATTA
ATATGTTCCCACTTTTTTCGCTAGCTATGAAAAAAAAAAGAATCACGTGACAATCGGACA
ATGTGACGTAACACAACTCTTTGAGATCTCTTTTATTCAGAGAGATAGTCTGATATTTTT
CAACGAGTAATGGAATAGTTTTCTTCAGCTAGCAAGAAATTATTATTTGCTTTTGTTGGT
CCATTTAAAC

Last Updated on 06.12.2018 16:11