Sequences from confirmation amplicon


Confirmation sequences for line 156F09 and hit At1g60987 are shown.
Line ID 156F09
Locus At1g60987 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:22460404
Primers used for PCR 49ms - 8474, show primer details
   
Sequence Name 17-K114404-0022-156-F09C-8409
GenBank Accession LN493949 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K114404-0022-156-F09C-8409
ATTGAATATATGAAAAACAAAAAATAGAAGAAACTTGCAAGAAAATATGAAGTTTGTTGC
AATCTTCTTGGTTACTTGTGTCTTATTCTCCCTTTTCCCAAGCCACCTGTCACAAGGTCT
TCTAATCATTCCACCGAAGTTACCATTTTGCTCATTGAAATTTGGTTGACCTAAACCAGT
TTGATTTAACTCTTTTAGTTGTTGTTGATCAATTAGGTGAAGAATCAAGGATGAATATTA
ATGCGGAGAGAAGGCCATGGTGTCCGTCTAAAATACAAATGTTTGATACTAATTGTGAAG
TGGATGGAGCAAAACAATGTCTAGATCTCTTAATAAGTACTTGGGATCCAAGTGTAAGGC
TCACCCGAGTCTCTNGCATTTGCTCTAATTTTCCTTACAGGAA
   
Sequence Name 39-K114405-0022-156-F09C-49ms
GenBank Accession LN503441 [GenBank]
Sequencing primer gene specific primer, 49ms: 5'-ATTCCTGTAAGGAAAATCAGAGCA-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K114405-0022-156-F09C-49ms
ATCCAAGTACTTATTAAGAGATCTAGACATTGTTTTGCTCCATCCACTTCACAATTAGTA
TCAAACATTTGTATTTTAGACGGACACCATGGCCTTCTCTCCGCATTAATATTCATCCTT
GATTCTTCACCTAATTGATCAACAACAACTAAAAGAGTTAAATCAAACTGGTTTAGGTCA
ACCAAATTTCAATGAGCAAAATGGTAACTTCGGTGGAATGATTAGAAGACCTTGTGACAG
GTGGCTTGGGAAAAGGGAGAATAAGACACAAGTAACCAAGAAGATTGCAACAAACTTCAT
ATTTTCTTGCAAGTTTCTTCTATTTTTTGTTTTTCATATATTCAATTGTAAATGGCTTCA
TGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC

Last Updated on 06.12.2018 16:11