Sequences from confirmation amplicon


Confirmation sequences for line 373A07 and hit At1g61140 are shown.
Line ID 373A07
Locus At1g61140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:22538759
Primers used for PCR DM24 - 8474, show primer details
   
Sequence Name 75-K101747-0022-373-A07C-8409
GenBank Accession LN513212 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K101747-0022-373-A07C-8409
TCGGAGTACTGATTCAAGCTGGCATTTGAGATTGAGAATCAATGCCCCTCGGTTGAATAT
ATTCCCGGTCTCCTTGTTGAATTTGAAAGTCTTTATACTCATTGTTTTGTGTCACTGGGC
TGGGATCTTCTACAACATATTGCAAACATGTATCTGAATCGTAAAAATGATTGGAGCTTA
TCTTGCCACTGTCTTGGCTAGGTAGAGCTGTTTGCGGTGCCATATATGAGTATCCTTTTT
CAATATCTGTTTGATAGGAGACTGCAGAGCTTGATGCACCAGACCACCAACGATTGGTTT
CTCCAGAAAGATAAGCATCATCCCTGACACTAAATTCCCCTGGAACTGAACTGTAGACAA
CTTTCTCTGATTCAGCCTCTGGTTTAACCTTCACATGAACAGTTTTATTATCCTGAAAAC
TGCATGAATAATTATTAGGCCGGTCAAGGTCACTATCAGCTAAATGACTGTCAAAACTGT
TGTAGGGTTTGACACAGGCAGGAGAAACTGAAGGATTACTTTCAAACTTGAAACTGATCC
CTCTTGCATAAGAAAAA
   
Sequence Name 75-K101753-0022-373-A07C-DM24
GenBank Accession LN513213 [GenBank]
Sequencing primer gene specific primer, DM24: 5'-ATTTTTCTAGTGCAAGAGGGATCA-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K101753-0022-373-A07C-DM24
TTTCTCCTGCCTGTGTCAACCCTACACAGTTTTGACAGTCATTTAGCTGATAGTGACCTT
GACCGGCCTAATAATTATTCATGCAGTTTTCAGGATAATAAAACTGTTCATGTGAAGGTT
AAACCAGAGGCTGAATCAGAGAAAGTTGTCTACAGTTCAGTTCCAGGGGAATTTAGTGTC
AGGGATGATGCTTATCTTTCTGGAGAAACCAATCGTTGGTGGTCTGGTGCATCAAGCTCT
GCAGTCTCCTATCAAACAGATATTGAAAAAGGATACTCATATATGGCACCGCAAACAGCT
CTACCTAGCCAAGACAGTGGCAAGATAAGCTCCAATCATTTTTACGATTCAGATACATGT
TTGCAATATGTTGTAGAAGATCCCAGCCCAGTGACACAAAACAATGAGTATAAAGACTTT
CAAATTCAACAAGGAGACCGGGAATATATTCAACCGAGGGGCATTGATTCTCAATTCTCA
AATGCCAGCTTTGAATCAGTTCACTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAAA
TATCCGCAGCGTTA

Last Updated on 06.12.2018 16:11